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当前位置: 首页 > 文章 > 皂荚种质资源RSAP遗传多样性分析及指纹图谱的构建 河南农业科学 2017 (11) 103-107
Position: Home > Articles > Genetic Diversity and Fingerprints of Glediysia sinensis Germplasms Based on RSAP Journal of Henan Agricultural Sciences 2017 (11) 103-107

皂荚种质资源RSAP遗传多样性分析及指纹图谱的构建

作  者：
范定臣;张安世;刘莹;骆扬
单  位：
河南省林业科学研究院
关键词：
皂荚;RSAP;DNA指纹图谱;遗传多样性
摘  要：
利用RSAP标记技术对18份皂荚种质材料进行了遗传多样性分析。结果表明,从45对RSAP引物中筛选了12对引物进行PCR扩增,共扩增出167个条带,其中多态性条带154个,多态性位点百分率(PPL)为92.22%。各引物Nei’s基因多样性指数(H)和Shannon’s信息指数(I)的平均值分别为0.229 8和0.371 6,18份皂荚种质的遗传相似系数(GS)为0.431 1~0.988 0。UPGMA聚类分析表明,在GS为0.62处可将18份皂荚种质资源分为3组,其中,野皂荚单独为一组,山皂荚和皂荚-T聚为一组,其他皂荚材料聚为一组。利用6对引物扩增的9个多态性位点,构建了18份皂荚种质资源的DNA指纹图谱,可以将其区分并精准鉴定。
译  名：
Genetic Diversity and Fingerprints of Glediysia sinensis Germplasms Based on RSAP
作  者：
FAN Dingchen;ZHANG Anshi;LIU Ying;LUO Yang;Henan Academy of Forestry;School of Science,Jiaozuo Teachers College;
单  位：
Henan Academy of Forestry%School of Science,Jiaozuo Teachers College
关键词：
Glediysia sinensis;;RSAP;;DNA fingerprint;;genetic diversity
摘  要：
Genetic diversity of 18 Glediysia sinensis germplasms was analyzed by the RSAP markers. The results showed that 12 primers were screened out of 45 primer pairs and 167 bands were obtained,including 154 polymorphic bands,with the percentage of polymorphic loci( PPL) of 92. 22%. The average Nei's gene diversity( H) and Shannon's information index( I) were 0. 229 8 and 0. 371 6,and the genetic similarity coefficients among the tested samples ranged from 0. 431 1 to 0. 988 0. UPGMA analysis showed that 18 Glediysia sinensis were clustered into 3 groups with the genetic similarity coefficient of0. 62. Glediysia heterophylla formed the first group,Glediysia melanacantha and Zaojia-T were clustered into the second group,and the others were clustered into the third group. In total of 18 Glediysia sinensis germplasm DNA fingerprints were constructed from 9 polymorphic loci amplified by 6 primer pairs and these materials could be distinguished and identified accurately.

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