作 者：
吕继洲;吴绍强;张永宁;王振宝;冯春燕;王彩霞;邓俊花;袁向芬;林祥梅
单 位：
新疆伊犁出入境检验检疫局;中国检验检疫科学研究院
关键词：
线粒体色素氧化酶亚基Ⅰ基因;16S rRNA;小亚璃眼蜱;亚洲璃眼蜱;残缘璃眼蜱;分子鉴定
摘 要：
本研究旨在建立小亚璃眼蜱、亚洲璃眼蜱和残缘璃眼蜱的分子生物学鉴定方法,并探讨它们的系统发生关系。在新疆、内蒙古从动物体表采集寄生蜱,形态学鉴定后,PCR扩增测序获得3种璃眼蜱的16SrRNA及线粒体色素氧化酶亚基Ⅰ基因(cytochrome oxidaseⅠ,COⅠ)序列后进行同源性分析。用Mega 5.0和Mrbayes 3.2软件分别构建系统进化树,亚洲璃眼蜱、小亚璃眼蜱样本16SrRNA序列与GenBank中已知相应蜱虫16SrRNA序列聚类,而残缘璃眼蜱COⅠ序列与GenBank中已知残缘璃眼蜱COⅠ序列聚类,与形态学鉴定结果一致。在传统形态学分类的基础上,结合分子生物学鉴定方法能简易、准确鉴定小亚璃眼蜱、亚洲璃眼蜱和残缘璃眼蜱。
译 名：
Molecular Identification of Hyalomma anatolicum,Hyalomma asiaticum and Hyalomma detritum
作 者：
LV Ji-zhou1,WU Shao-qiang1,ZHANG Yong-ning1,WANG Zhen-bao2,FENG Chun-yan1,WANG Cai-xia1,DENG Jun-hua1,YUAN Xiang-fen1,LIN Xiang-mei1(1.Institute of Animal Quarantine,Chinese Academy of Inspection and Quarantine,Beijing 100029,China;2.Yili Entry-exit Inspection and Quarantine Bureau,Yili 835000,China)
关键词：
cytochrome oxidaseⅠ;16S rRNA;Hyalomma anatolicum;Hyalomma asiaticum;Hyalomma detritum;molecular identification
摘 要：
This study is to establish a method for identifying Hyalomma anatolicum(H.anatolicum),Hyalomma asiaticum(H.asiaticum)and Hyalomma detritum(H.detritum)with molecular markers and to learn the phylogenetic relationship of these ticks.Ticks were collected from domestic animals in Xinjiang and Inner Mongolia provinces and classified by morphology characters.Then the 16S rRNA and COⅠ of ticks were amplified by PCR and subsequently sequenced.Phylogenetic trees were constructed by Mega 5.0 and Mrbayes 3.2.On the phylogenetic trees based on the 16S rRNA,H.anatolicum and H.aisaticum were clustered together with their respective classes.And H.detritum was clustered with their respective class and the H.anatolicum,H.asiaticum formed distinct branches on the phylogenetic trees based on the COⅠ.The method based on morphology that combined with molecular 16S rRNA and COⅠ seemed a simple,accuracy method for the identification of H.anatolicum,H.asiaticum and H.detritum.
