单 位：
新疆伊犁出入境检验检疫局;中国检验检疫科学研究院动物检疫所
关键词：
线粒体色素氧化酶亚基Ⅰ基因;16S rRNA;草原革蜱;边缘革蜱;分子鉴定
摘 要：
本研究旨在建立草原革蜱和边缘革蜱的分子生物学鉴定方法,并探讨其系统发生关系。在新疆从动物体表采集寄生蜱,形态学鉴定后,PCR扩增获得2种革蜱的16S rRNA及线粒体色素氧化酶亚基Ⅰ(COⅠ)基因序列,测序后进行同源性分析。用Mega 5.0和Mrbayes 3.2软件分别构建系统进化树,草原革蜱16S rRNA序列与GenBank数据库中已知草原革蜱16S rRNA序列聚类,而边缘革蜱COⅠ序列与GenBank数据库中已知边缘革蜱COⅠ序列聚类,与形态学鉴定结果一致。本研究结果表明,在传统形态学分类的基础上,结合分子生物学鉴定方法能简易、准确鉴定草原革蜱和边缘革蜱。
译 名：
Molecular Biological Identification of Dermacentor nuttalli and Dermacentor marginatus
作 者：
LV Ji-zhou;WANG Zhen-bao;YUAN Xiang-fen;WU Shao-qiang;Institute of Animal Quarantine,Chinese Academy of Inspection and Quarantine;Yili Entry-exit Inspection and Quarantine Bureau;
关键词：
COⅠ;;16S rRNA;;Dermacentor nuttalli;;Dermacentor marginatus;;molecular identification
摘 要：
This study was to establish a method for identifying Dermacentor nuttalli(D.nuttalli) and Dermacentor marginatus( D. marginatus) with molecular markers,and study the phylogenetic relationship of these two species of ticks. Ticks were collected from domestic animals in Xinjiang province and classified by morphology characters. Then 16S rRNA and COⅠ of ticks were amplified by PCR and subsequently sequenced. Phylogenetic trees were constructed by Mega 5. 0 and Mrbayes 3. 2. On the phylogenetic trees based on 16S rRNA,D. nuttalli was clustered together with their respective class D. nuttalli,and D. marginatus formed a distinct branch. Moreover,D. marginatus was clustered with their respective class D. marginatus,and D. nuttalli formed a distinct branch on the phylogenetic trees based on COⅠ. Based on morphology and combined with molecular biology identification,the method was a simple and accurate method for the identification of D. nuttalli and D. marginatus.
