作 者：
刘玉娇;宋乐天;郭书欣;卢珊;王德才;李艳玲
单 位：
山东第一医科大学(山东省医学科学院)生命科学学院;山东大学药学院;山东第一医科大学(山东省医学科学院)药学院
关键词：
丹参种子;植物内生菌;生物活性;抑菌;抗氧化;抗凝血
摘 要：
目的:从丹参种子中分离纯化内生菌,筛选具有抑菌、抗氧化、抗凝血活性的菌株进行分子鉴定。方法:采用平板分离法从丹参种子中分离内生菌;采用平板对峙法和滤纸片法筛选具有抑菌效果的内生菌,采用DPPH法测定内生菌抗氧化活性,兔耳缘静脉取血测定内生菌抗凝血活性。结果:从白花丹参种子中分离出11株内生真菌和2株内生细菌,从紫花丹参种子中分离出9株内生真菌和9株内生细菌。其中,一株内生真菌ZJZD-3发酵液对丹参病原菌4和6均有抑制作用,抑制率分别为70.1%和59.6%;内生真菌ZJZD-3具有很好的抗氧化能力,其0.5 mg/mL乙醇粗提液对DPPH清除率为91.44%;内生真菌ZJZD-3对于内源性、外源性凝血途径都具有一定的延长作用,并且内源性凝血途径最为明显,对家兔凝血酶时间(TT)、凝血酶原时间(PT)、活性部分凝血酶时间(APTT)的延长率分别为48.48%、12.38%和34.44%。该菌株经鉴定为篮状菌属(Talaromyces sp.)。结论:内生真菌ZJZD-3具有很好的抗丹参病原菌、抗氧化和抗凝血能力,为丹参药效成分的工业化开发以及丹参的绿色规模化栽培提供新的途径和方法。
译 名：
Isolation,Identification and Biological Activity of an Endophytic Fungus Strain ZJZD-3 from Salvia miltiorrhiza Seed
作 者：
LIU Yu-jiao;SONG Le-tian;GUO Shu-xin;LU Shan;WANG De-cai;LI Yan-ling;College of Life Sciences,Shandong First Medical University& Shandong Academy of Medical Sciences;College of Pharmacy,Shandong University;College of Pharmacy,Shandong First Medical University & Shandong Academy of Medical Sciences;
关键词：
Salvia miltiorrhiza seed;;endophytes;;bioactivity;;anti-phytopathogenic fungi;;antioxidant;;anticoagulant
摘 要：
Objective: To isolate,purify and screen endophytes from Salvia miltiorrhiza seeds,which can inhibit plant pathogenic fungi and possess antioxidant and anticoagulant activities. Methods: Endophytes were isolated from seeds of Salvia miltiorrhiza by the plate method,the endophytic fungi which had antifungal effects were isolated by the method of plate confrontation and filter paper.The DPPH method was used to measure the antioxidant activity.The anticoagulation activity of endophytic bacteria was measured in the peripheral vein of rabbit ear. Results: 11 endophytic fungi and 2 endophytic bacteria were isolated from seeds of Salvia miltiorrhiza. 9 endophytic fungi and 9 endophytic bacteria were isolated from seeds of Salvia miltiorrhiza.Among them,a strain of endophytic fungus numbered as ZJZD-3 could restrain the pathogenic fungi No.4 and No.6 of Salvia miltiorrhiza,its inhibition rate was 70.1% and 59.6%,respectively.And the ZJZD-3 had the strongest antioxidant capacity,the DPPH scavenging rate was 91.44% with 0.5 mg/m L ethanol crude extract of ZJZD-3.In addition,ZJZD-3 had extended effect on intrinsic and exogenous coagulation,especially on intrinsic coagulation.The extension rates of ZJZD-3 to rabbits TT,PT and APTT were 48.48%,12.38% and 34.44%,respectively. The ZJZD-3 was identified as Talaromyces. Conclusions: Endophytic fungus ZJZD-3 has good ability of inhibiting plant pathogenic fungi,antioxidant and anticoagulant.It can improve new ways and methods for the development of Salvia miltiorrhiza resources and high-quality and high-yield cultivation.
