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Position: Home > Articles > Study on determination of oligo-chitosan by thin layer chromagraphy Science and Technology of Food Industry 2008 (4) 271-273

一种新型壳低聚糖检测方法的研究

作  者:
王晓燕;许牡丹
单  位:
陕西科技大学生命学院
关键词:
壳低聚糖;TLC法
摘  要:
实验结果表明,用展开剂乙酸乙酯:无水乙醇:水:氨水=5:5:4:0.45,显色剂为苯胺-二苯胺-磷酸,点样量为2μg/μL,可将壳低聚糖单糖至六糖各组分分开,斑点清晰,圆形,无拖尾,分离效果好。以薄层-比色法检测壳低聚糖的含量,具有样品回收率高,相对误差较小,方法简便,准确性高等优点,适合于壳低聚糖常规检验。
译  名:
Study on determination of oligo-chitosan by thin layer chromagraphy
作  者:
WANG Xiao-yan,XU Mu-dan(Institute of Life Sciences and Engineering,Shaanxi University of Science & Technology,Xi'an 710021,China)
关键词:
oligo-chitosan;thin layer chromagraphy
摘  要:
The determination of oligo-chitosan was studied by thin layer chromagraphy.The suitable parameter was gotten when developing agent was ethyl acetate∶alcohol∶water∶ammonia water=5∶5∶4∶0.45,chromogenic agent was aniline-diphenylamine-phosphricacid.It was convenient and accurate and this way was proved to be dependable.

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