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超声波提取翻白草中熊果酸工艺优化研究

作  者:
李胜华;伍贤进;郁建平;王丽娜;胡美忠
单  位:
怀化学院生物工程系;贵州大学生化营养研究所
关键词:
翻白草;熊果酸;提取
摘  要:
目的:筛选翻白草中熊果酸的最佳提取工艺。方法:采用超声波提取、正交试验优化及高效液相色谱法测定所得翻白草中熊果酸的含量。结果:翻白草中熊果酸的提取最佳工艺是乙醇浓度为75%,固液比为1:60,提取时间为60min,超声波功率为610W,各因素的影响依次为提取时间>固液比>乙醇浓度和超声波功率。结论:最佳优选结果用于验证实验具有较好的重现性。
译  名:
Study on Optimization of Extraction Process of Ursolic Acid from Potentilla discolor Bge with Ultrasonic
作  者:
LI Sheng-hua1,WU Xian-jin1,*,YU Jian-ping2,WANG Li-na1,HU Mei-zhong2 (1.Department of Bioengineering, Huaihua College, Huaihua 418008, China; 2.Institute of Biochemistry and Nutrient, Guizhou University, Guizhou 550025, China)
关键词:
Potentilla discolor Bge.;ursolic acid;extraction
摘  要:
Objective: To screen optimum the extraction process of ursolic acid from Potentilla discolor Bge. Methods: ultrasonics extraction was used to extract. The optimum extraction process was confimed by the orthogonal test. The content of ursolic acid in Potentilla discolor Bge. was determined by HPLC. Results: The optimum conditions of ultrasonics extraction are as follow: ethanol concentration 75%, time 60 min, ratio of material to solution 1:60, and power of extraction 610 W. The effects of factors on yield decrease progressively in the following order: extracting time>ratio of material to solution>ethanol concentration>power of extraction. Conclusion: The best optimized process has a good repeatability confimatory experiment.

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