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Position: Home > Articles > The In Vitro Bacteriostatic Activity of Sanguinarine on Saprolegnia sp Journal of Mountain Agriculture and Biology 2012,31 (5) 450-453

血根碱抗水霉菌体外活性研究

作  者:
王小玲;郁建平;范家佑
单  位:
贵州大学生命科学学院
关键词:
水霉菌;血根碱;抑菌活性;氯化钠;二甲亚砜
摘  要:
采用测OD值和琼脂稀释法,以孔雀石绿、氯化钠作为对照,分别进行了3种药物对水霉菌孢子和菌丝的体外抑菌活性研究,其中血根碱以2%DMSO(二甲亚砜)为溶剂,孔雀石绿和NaCl均以水为溶剂。结果表明:血根碱对水霉孢子的MIC80为200μg/mL,对菌丝的MIC为256μg/mL;孔雀石绿对水霉菌孢子的MIC80为6μg/mL,对菌丝的MIC为12μg/mL;氯化钠对水霉菌孢子的MIC80为15 000μg/mL,对菌丝的MIC为20 000μg/mL,说明血根碱具有开发为抗水霉菌药物应用于渔药领域的前景。
译  名:
The In Vitro Bacteriostatic Activity of Sanguinarine on Saprolegnia sp
作  者:
WANG Xiao-ling,YU Jian-ping,FAN Jia-you(College of Life Sciences,Guizhou University,Guiyang Guizhou 550025,China)
关键词:
Saprolegnia;Sanguinarine;antibacterial activity;Sodium chloride;Dimethyl sulfoxide
摘  要:
With malachite green and sodium chloride diluted in water as control,the In Vitro activity of sanguinarine to spores and mycelium of Saprolegnia diluted with 2% dimethyl sulfoxide was measured by OD test.The result revealed that the MIC of sanguinarine against Saprolegnia sp.spores and mycelium were 200 μg/mL and 256 μg/mL,respectively.MIC of malachite green or sodium chloride against Saprolegnia sp.spores and mycelium were 6 μg/mL and 12 μg/mL or 15 000 μg/mL and 20 000 μg/mL,respectively.Based on the above mentioned results,it may be concluded that sanguinarine shows a potential to be developed as a new fishery drug to control Saprolegniasis sp..

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