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当前位置: 首页 > 文章 > 甘草次酸对斑点叉尾鮰免疫应激下血浆抗氧化酶和免疫指标的影响 水产学报 2016,40 (9) 1368-1375
Position: Home > Articles > Effect of glycyrrhetinic acid(GA) on the physiological responses and immunoreactions of channel catfish(Ictalurus punctatus)injection lipopolysaccharide(LPS) Journal of Fisheries of China 2016,40 (9) 1368-1375

甘草次酸对斑点叉尾鮰免疫应激下血浆抗氧化酶和免疫指标的影响

作  者：
蒋广震;周嫚;李向飞;张定东;刘文斌
单  位：
南京农业大学动物科技学院江苏省水产动物营养重点实验室
关键词：
斑点叉尾鮰;甘草次酸;脂多糖;免疫应激;抗氧化酶活性
摘  要：
为探讨斑点叉尾鮰免疫应激状态下血浆抗氧化酶和免疫指标的变化,并研究甘草次酸对斑点叉尾鮰免疫应激的调控机制,实验设计两组,每组6个重复:1)对照组,饲喂基础饲料;2)GA组,饲喂含0.15 g/kg甘草次酸饲料,养殖8周后,腹腔注射大肠杆菌脂多糖(LPS),分别在注射后0、3、6、12、24、48 h采集血液和肝脏组织,进行红细胞、白细胞计数,分离血浆后检测血浆史质醇、溶菌酶、ACH50、谷草转氨酶、谷丙转氨酶、碱性磷酸酶活性,肝脏匀浆液检测SOD、MDA和CAT抗氧化酶活性。结果显示,在腹腔注射LPS后,血浆溶菌酶、史质醇、谷草转氨酶、谷丙转氨酶、ACH50、SOD均呈现先升高后下降的趋势,白细胞计数、血浆CAT呈现先下降后升高的趋势;饲料中添加0.15 g/kg甘草次酸显著抑制了各指标变化,缩短了由LPS诱导产生各指标变化的恢复时间。研究表明,腹腔注射LPS使斑点叉尾鮰产生免疫应激,并在注射后6~12 h达到顶峰;饲料中添加0.15 g/kg甘草次酸可以有效抑制由LPS诱导的免疫应激反应。
译  名：
Effect of glycyrrhetinic acid(GA) on the physiological responses and immunoreactions of channel catfish(Ictalurus punctatus)injection lipopolysaccharide(LPS)
作  者：
JIANG Guangzhen;ZHOU Man;LI Xiangfei;ZHANG Dingdong;LIU Wenbin;Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province,College of Animal Science and Technology,Nanjing Agricultural University;
关键词：
Ictalurus punctatus;;glycyrrhetinic acid;;lipopolysacchande;;immunological stress;;antioxidant enzyme activity
摘  要：
Glycyrrhetinic acid(GA),which is a main active principal constituent of liquorice,is extensively used as antibacterial and anti-inflammatory Chinese medicine.This study was carried out to evaluate the effects of GA on the oxidative stress of Ictalurus punctatus injection lipopolysaccharide(LPS).The fish were randomly divided into two groups:a control group(fed a standard diet) and a treatment group(standard diet supplemented with 0.15mg/kg GA) and fed for 8 weeks.We then challenged the fish with LPS and recorded changes in red and white blood cell counts(RBC and WBC),plasma Cortisol,lysozyme,ACH50,aspartate aminotransferase(AST),alanine aminotransferase(ALT) and hepatic catalase(CAT),superoxide dismutase(SOD) and malondialdehyde(MDA)for a period of 48 h.Supplementation with 0.15 g/kg GA significantly increased plasma lysozyme activity before infection,plasma ACH50 activity at 6 h after infection,WBC hepatic at 6 h after infection,CAT activity 24 h after infection,hepatic SOD activity before and after infection.In addition,the supplemented group had decreased levels of plasma AST and ALT activities 6 h after infection,hepatic CAT 3 h after infection,hepatic MDA 24 and48 h after infection.Our results suggest that ingestion of a basal diet supplemented with 0.15 g/kg GA prevents LPS-induced immunological challenges response by regulating key enzyme activities,and challenged with LPS(2.0 mg/kg) affect physiological responses and immunoreactions of channel catfish.

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