当前位置: 首页 > 文章 > 生防细菌L5生防相关因子的初步分析及其种类鉴定 植物病理学报 2011,41 (3) 295-300
Position: Home > Articles > Characterization and identification of a biocontrol bacterial strain L5 Acta Phytopathologica Sinica 2011,41 (3) 295-300

生防细菌L5生防相关因子的初步分析及其种类鉴定

作  者:
钱兰娟;李倩;张清霞;童蕴慧;徐敬友
单  位:
扬州大学园艺与植物保护学院
关键词:
16S rDNA;16S-23S rDNA间区;沙雷氏菌;硝吡咯菌素;番茄灰霉病菌
摘  要:
从扬州郊区土壤中分离到细菌菌株L5,该菌株抑菌谱广,对多种病原真菌都有明显的拮抗能力,离体叶片生物测定结果表明菌株L5对番茄灰霉病防效达72%。对该菌株的生防相关性状进行分析,结果表明菌株L5可产生硝吡咯菌素(Pyrrolnitrin)和嗜铁素等抗菌物质。经过16S rDNA序列、16S-23S rDNA间区序列同源性分析和生理生化性状的测定,将该菌株鉴定为气味沙雷氏菌(Serratia odorifera)。细菌菌株L5包含2个16S-23S rDNA的间区(IGSs),其中IGS1内含tRNAGlu基因,间区类型属于IGSG;IGS2内含tRNAIle和tRNAAla基因,间区类型属于IGSIA。
译  名:
Characterization and identification of a biocontrol bacterial strain L5
作  者:
QIAN Lan-juan,LI Qian,ZHANG Qing-xia,TONG Yun-hui,XU Jing-you(College of Horticulture and Plant Protection,Yangzhou University,Yangzhou 225009,China)
关键词:
16S rDNA;16S-23S rDNA IGS;Serratia;pyrrolnitrin;Botrytis cinerea
摘  要:
Bacterial strain L5 was isolated from soil in Yangzhou and showed inhibitory effect to many pathogenic fungi in dual-culture tests.This strain produced pyrrolnitrin and siderphore,and showed 72% efficiency to control grey mould caused by Botrytis cinerea on detached tomato leaves.The strain L5 was identified as Serratia odorifera based on sequence analysis of 16S rDNA and 16S-23S rDNA intergenic spacers(IGS),physiological and biochemical characterization.The analysis of IGS sequence found two different types of IGS in strain L5.One included the tRNAGlu,and the other contained a cluster of tRNAIle and tRNAAla.

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