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Position: Home > Articles > Determination of Silybin Content in Chinese Herb Compound "Jiangansan" by HPLC Progress in Veterinary Medicine 2013,34 (1) 47-50

中草药复方“健肝散”中水飞蓟宾含量的高效液相色谱法测定

作  者:
张盈娇;曹阳;夏陈;曾晓丹;古锐
单  位:
成都中医药大学;四川省农业科学院农产品加工研究所
关键词:
中草药复方;健肝散;水飞蓟宾;高效液相色谱
摘  要:
采用十八烷基硅烷键合硅胶柱Welchrom-C18(4.6mm×250mm,5μm)为色谱柱,柱温30℃,流动相为乙腈-甲醇-5mL/L醋酸溶液(体积比为16∶26∶58),流速为1.00mL/min,检测波长287nm,进样量10μL。结果表明,水飞蓟宾的进样量在0.219μg~5.475μg(r=0.999 999,n=6)范围内与其峰面积积分值呈良好的线性关系,平均加样回收率为101.211%(n=6)。表明建立的高效液相色谱法快速、准确,重现性和精密度均良好,可为中草药复方"健肝散"的质量控制和评价提供技术支撑。
译  名:
Determination of Silybin Content in Chinese Herb Compound "Jiangansan" by HPLC
作  者:
ZHANG Ying-jiao1,CAO Yang1,XIA Chen1,ZENG Xiao-dan1,GU Rui2(1.Sichuan Academy of Agricultural Sciences,Chengdu,Sichuan,610066,China; 2.Chengdu University Of TCM,Chengdu,Sichuan,610072,China)
关键词:
Chinese herb compound;Jiangansan;silybin;HPLC
摘  要:
To establish a HPLC method for determination of silybin in "Jiangansan" of veterinary drug,the content determination was performed on Welchrom-C18(4.6 mm×250 mm,5 μm),with the mobile phase consisted of acetonitrile-methanol-5 mL/L acetic acid(16∶26∶58).The flow rate was 1.0 mL/min,and the detection wavelength of silybin was set at 287 nm.The column temperature was maintained at 30 ℃ and injection volume was 10 μL.In result,the linear range of silybin was from 0.219 to 5.475 μg(r=0.999 999),and the average recovery were 101.211%.In conclusion,the method was simple,accurate,reproducible and precise,which would be suitable for content determination,quality control and evaluation of silybin in "Jiangansan".

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