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当前位置: 首页 > 文章 > 旋毛虫细胞外囊泡对TNBS诱导的小鼠实验性结肠炎的初步干预作用 动物医学进展 2020 (5) 67-73
Position: Home > Articles > Preliminary Prevention Effect of Trichinella spiralis Extracellular Vesicles on TNBS-Induced Experimental Colitis in Mice Progress in Veterinary Medicine 2020 (5) 67-73

旋毛虫细胞外囊泡对TNBS诱导的小鼠实验性结肠炎的初步干预作用

作  者：
刘蕾;杨勇;高欣;刘晓雷;刘明远;徐凝;白雪
单  位：
吉林大学人兽共患病研究所/人兽共患病研究教育部重点实验室/动物医学学院
关键词：
旋毛虫;细胞外囊泡;2,4,6-三硝基苯磺酸;实验性结肠炎
摘  要：
为探讨旋毛虫细胞外囊泡(Trichinella spiralis extracellular vesicles,Ts-EVs)对2,4,6-三硝基苯磺酸(TNBS)诱导的小鼠实验性结肠炎的初步干预作用,通过超速离心法获得Ts-EVs,利用透射电子显微镜(TEM)对其进行鉴定。随后将30只Balb/c小鼠随机分为正常对照组、TNBS模型组和TNBS+Ts-EVs干预组。通过测定各组小鼠肠道疾病活动指数(DAI)、髓过氧化物酶(MPO)活性和结肠病理组织学变化等指标评价Ts-EVs对结肠炎的干预效果;进一步用ELISA检测外周血血清中细胞因子的表达情况。TEM结果显示,EVs具有膜结构,形态呈圆形或椭圆形,平均直径为30nm～150nm;评估Ts-EVs对TNBS诱导的小鼠结肠炎的干预效果表明,TNBS+Ts-EVs干预组小鼠的结肠炎临床症状有所缓解,炎症指标均明显降低;与TNBS模型组相比,TNBS+Ts-EVs干预组小鼠的Th1细胞因子(IL-1β、IFN-γ)和Th17细胞因子(IL-17A)表达水平均显著降低;而Treg细胞因子(IL-10、TGF-β)表达水平明显升高。研究结果表明Ts-EVs可有效干预TNBS诱导的小鼠实验性结肠炎,此干预作用可能与调节Th细胞免疫平衡有关。
译  名：
Preliminary Prevention Effect of Trichinella spiralis Extracellular Vesicles on TNBS-Induced Experimental Colitis in Mice
作  者：
LIU Lei;YANG Yong;GAO Xin;LIU Xiao-lei;LIU Ming-yuan;XU Ning;BAI Xue;Institute of Zoonosis,Key Laboratory of Zoonosis Research,Ministry of Education,College of Veterinary Medicine,Jilin University;
关键词：
Trichinella spiralis;;extracellular vesicle;;2,4,6-trinitrobenzene sulfonic acid;;experimental colitis
摘  要：
To investigate the preliminary prevention effect of Trichinella spiralis extracellular vesicles(TsEVs)on 2,4,6-trinitrobenzene sulfonic acid(TNBS)-induced experimental colitis in mice,Ts-EVs were first obtained by ultracentrifugation and identified by transmission electron microscopy(TEM).30 Balb/c mice were then randomly divided into control group,TNBS group and TNBS+Ts-EVs group.Intestinal disease activity index(DAI),body weight change,myeloperoxidase(MPO)activity and lais to histopathological lesions of colon were measured in each group to evaluate the prevention effect of Ts-EVs in colitis;ELISA was used to detect the expression of cytokines in serum.TEM results showed that EVs had a membrane structure and a circular or elliptical shape with an average diameter of 30 nm-150 nm.The evolution of the prevention effect of Ts-EVs on TNBS-induced colitis in mice showed that the clinical symptoms of colitis in the TNBS+Ts-EVs group were alleviated,and the inflammation indexes were significantly reduced.The ELISA results showed that the expression levels of Th1 cytokines(IL-1β,IFN-γ)and Th17 cytokine(IL-17 A)were significantly lower in the TNBS+Ts-EVs group,while the expression levels of regulatory cytokines(IL-10,TGF-β)were significantly increased compared with the TNBS group.The results of this study indicated that Ts-EVs can effectively alleviate TNBS-induced colitis in mice.This prevention effect may be related with the regulation of the blance of Th cell immune resopones.

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