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Position: Home > Articles > RAPD Analysis of Arrowroot( Pueraria L. ) Germplasm Genetic Relationship Crop Research 2013 (4) 347-350

葛种质资源亲缘关系的RAPD分析

作  者:
周精华;揭雨成;杜晓华;邢虎成;熊力夫
单  位:
长沙市葛根工程技术研究中心;湖南农业大学苎麻研究所
关键词:
葛;种质资源;亲缘关系;RAPD
摘  要:
利用RAPD分子标记技术对来自湖南和江西的8份葛种质资源进行亲缘关系分析。从100个随机引物中筛选出10个多态性较好的引物,共扩增出99条带,多态性条带所占比例为65.65%。聚类分析结果表明:8份材料的遗传相似系数在0.626~0.939之间;在遗传相似系数0.740处可将8份材料分为4类,其中E1、E2、E7和E8聚为一类,表明这些材料亲缘关系较近;葛所属类群与地理来源有关。
译  名:
RAPD Analysis of Arrowroot( Pueraria L. ) Germplasm Genetic Relationship
作  者:
ZHOU Jing-hua1,JIE Yu-cheng1,2*,DU Xiao-hua,XING Hu-cheng1,2,XIONG Li-fu3(1 Institute of Ramie,Hunan Agricultural University,Changsha,Hunan 410128,China;2 Hunan Provincial Key Laboratory of Corp Germplasm Innovation and Utilization,Changsha,Hunan 410128,China;3 Changsha Arrowroot Engineering Technology Research Center,Changsha,Hunan 410005,China)
关键词:
Arrowroot(Pueraria L.);Germplasm resources;Genetic Relationship;RAPD
摘  要:
In this study,the genetic relationships of 8 arrowroots that come from Hunan and Jiangxi province were analyzed by RAPD(random amplified polymorphic DNA) molecular marker technology.The results showed that a total of 99 bands were amplified by 10 primers which had high polymorphism selected from 100 random primers,with the percentage of polymorphic bands of 65.65%.The results of cluster analysis revealed that the value of genetic similarity coefficient of 8 materials changed from 0.626 to 0.939.The 8 materials could be classified into 4 groups at coefficient of 0.740 and E1、 E2、E7、E8 were gathered in the 4th group,indicating that these materials shared closer genetic relationship.This conclusion was effected by the original and provided references for the introduction,cultivation and improment of arrowroot.

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