当前位置: 首页 > 文章 > 指纹图谱条件下何首乌中二苯乙烯苷的含量测定 山地农业生物学报 2012,31 (3) 79-82
Position: Home > Articles > Determination of stilbene glucoside in Polygonum multiflorum Thunb by HPLC fingerprint Journal of Mountain Agriculture and Biology 2012,31 (3) 79-82

指纹图谱条件下何首乌中二苯乙烯苷的含量测定

作  者:
李燕;王慧娟;林冰;赵致;周英
单  位:
贵州大学生命科学学院
关键词:
二苯乙烯苷;含量测定;指纹图谱;何首乌
摘  要:
采用高效液相色谱仪,在指纹图谱条件下测定了20批何首乌药材中二苯乙烯苷的含量。所选色谱柱Ag-ilent SB-C18(5μm,4.6 mm×250 mm),检测波长280 nm,流动相乙腈-0.4%磷酸水溶液梯度洗脱,流速1.0mL/min,柱温25℃,分析时间60 min。经精密度、稳定性、重复性试验考察,RSD均小于3%,平均回收率为100.33%,表明所建立的方法稳定可靠,可满足企业在进行指纹图谱研究的同时进行二苯乙烯苷的含量测定。
译  名:
Determination of stilbene glucoside in Polygonum multiflorum Thunb by HPLC fingerprint
作  者:
LI Yan1,2,WANG Hui-Juan1,2,LIN Bing1,2,3,ZHAO Zhi1,3,ZHOU Ying1,2,3(1.College of Life Sciences,Guizhou University,Guiyang Guizhou 550025,China;2.Research and Development Center of Chinese Herbal Medicine and Natural Medicine of Guizhou University,Guiyang Guizhou 550025,China;3.Key Laboratory of Chinese Herbal Medicine Planting and Breeding of Guizhou Province,Guiyang Guizhou 550025,China)
关键词:
Stilbene glucoside;content determination;fingerprint chromatogram;Polygonum multi-florum Thunb
摘  要:
The stibene glycosides content was determined in 20 samples of Polygonum multiflorum Thunb under the conditions of HPLC fingerprint.The method was established as follows: Chromatographic column Agilent SB-C18(5μm,4.6mm×250mm);Detective wavelength 280nm;Mobile phase acetonitrile-0.4% water solution of phosphori acid graditent elution;velocity 1.0mL/min;Column temperature 25 degree centigrade;analysis time 60min.The RSD were all less than 3% in precision,stability and repeatability testing study,and the average recovery rate was 100.33%.It indicated that the established method was stable and reliable,which made it possible for enterprises to determine the content of stilbene glucoside while studying fingerprints.

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