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Position: Home > Articles > Determination of Sugars in Infant Formula by Ion Chromatography Journal of Dairy Science and Technology 2020 (1) 9-14

离子色谱法测定婴幼儿配方乳粉中的糖类

作  者：
李莉;曹文军;薛伟锋;刘明
单  位：
大连市检验检测认证技术服务中心;大连海关技术中心;中检(大连)测试技术有限公司
关键词：
离子色谱;脉冲安培检测;梯度洗脱;婴幼儿配方乳粉;多种糖类
摘  要：
为建立操作简便、快速有效、同时分析婴幼儿配方乳粉中多种糖类的离子色谱分析方法,采用高效阴离子交换柱分离-脉冲安培检测法建立婴幼儿配方乳粉中葡萄糖、蔗糖、乳糖、低聚半乳糖三糖、蔗果三糖、低聚半乳糖五糖、蔗果四糖和蔗果五糖的分析方法。考察色谱柱、柱温、检测波形和淋洗液(NaOH、NaAc)浓度及流速对8种糖分离效果的影响。结果表明:选用CarboPac PA20阴离子交换柱,包括分析柱(3 mm×150 mm)和保护柱(3 mm×30 mm),流速0.30 mL/min,柱温30℃,在选定的流动相梯度洗脱程序条件下实现高效分离;本方法对葡萄糖、蔗糖、乳糖、低聚半乳糖三糖、蔗果三糖、低聚半乳糖五糖、蔗果四糖和蔗果五糖的检出限为3.0～12.0μg/L,精密度实验相对标准偏差小于3%,豆浆样品中8种糖类的加标回收率为78.0%～96.8%,表明该方法可行。
译  名：
Determination of Sugars in Infant Formula by Ion Chromatography
作  者：
LI Li;CAO Wenjun;XUE Weifeng;LIU Ming;Dalian Inspection and Certification Technology Service Center;China Inspection (Dalian) Test Technology Co.Ltd.;Dalian Customs Technology Center;
关键词：
ion chromatography;;pulsed amperometric detection;;gradient elution;;infant formula;;a variety of sugars
摘  要：
The purpose of this study is to establish a simple, rapid and effective method for the simultaneous analysis of various saccharides(glucose, sucrose, lactose, galactooligotrisaccharide, kestose, galactooligopentasaccharide, nystose and 1 F-fructofuranosyl nystose) in infant formula by high performance anion exchange chromatography with pulsed amperometric detection(HPAEC-PAD). The effect of chromatographic columns, column temperature, detector waveforms, eluent concentration and flow rate on the separation of the 8 saccharides was evaluated. The analysis was performed on a CarboPac PA20 analytical column(3 mm × 150 mm) coupled with a guard column(3 mm × 30 mm) at a 0.30 mL/min flow rate. Column temperature was set as 30 ℃. Efficient separation was achieved using gradient elution. Under the optimal conditions, the detection limit was 3.0–12.0 μg/L for all analytes. The precision, expressed as relative standard deviation(RSD), was less than 3%. The recoveries of the 8 saccharides in spiked soymilk were 78.0%–96.8%, indicating the feasibility of this method.

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