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Position: Home > Articles > Determination of Melamine in Liquid Milk by Ion Chromatography with UV Detection FOOD SCIENCE 2009,30 (14) 240-242

离子色谱-紫外检测测定液态奶中的三聚氰胺

作  者:
林立;王海波;梁立娜
单  位:
国家食品质量安全监督检验中心;Dionex(戴安)中国有限公司应用研究中心
关键词:
三聚氰胺;离子色谱;液态奶制品
摘  要:
本实验建立了液态奶制品中三聚氰胺的离子色谱-紫外检测测定方法。样品经乙腈沉淀蛋白后,将上清液离心过滤,稀释后过OnGuardRP柱除去样品中的脂肪,滤液进样至离子色谱,以IonPacCS17分析柱进行阳离子交换分离。三聚氰胺的含量在0.02~5.0mg/L浓度范围内,工作曲线方程为Y=1.8697X-0.0050,线性相关系数r为0.99995。利用加标回收实验考察了方法的准确性,回收率在82%~94%的范围之内。该方法方便可靠,检出限为0.03mg/kg,可应用于液态奶制品中三聚氰胺的检测。
译  名:
Determination of Melamine in Liquid Milk by Ion Chromatography with UV Detection
作  者:
LIN Li1,WANG Hai-bo2,LIANG Li-na2 (1. China National Food and Safery Supervision and Inspection Center, Beijing 100094, China; 2. Application Research Center, Dionex China Co. Ltd., Beijing 100085, China)
关键词:
melamine;ion chromatography;liquid milk
摘  要:
An analytical method using ion chromatography separation coupled with UV detection was developed for the determination of melamine in liquid milk. The procedure was based on extraction of test portions with acetonitrile, cleanup on an OnGuard II RP column to remove fat and filtration through 0.22 μm nylon membrane followed by ion chromatographic analysis of the extracts, using an IonPac CS17 column and UV detection at 240 nm. The calibration curve showed good linearity in the range of 0.02-5.0 mg/L, with a correlation coefficient of 0.99995. The recoveries for melamine spiked liquid milk ranged 82% -94%. The method has the advantages of easiness and quickness, and therefore can be applied for the determination of melamine in liquid milk.

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