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当前位置: 首页 > 文章 > A genetic evidence of chromosomal fragment from bridge parent existing in substitution lines between two common wheat varieties 农业科学学报 (英文) 2016,15 (1)
Position: Home > Articles > A genetic evidence of chromosomal fragment from bridge parent existing in substitution lines between two common wheat varieties Journal of Integrative Agriculture 2016,15 (1)

A genetic evidence of chromosomal fragment from bridge parent existing in substitution lines between two common wheat varieties

作  者：
Zhao Pei;Wang Ke;Lin Zhi-shan;Liu Hui-yun;Li Xin;Du Li-pu;Yan Yue-ming;Ye Xing-guo
单  位：
Capital Normal Univ, Coll Life Sci, Key Lab Genet & Biotechnol, Beijing 100048, Peoples R China;Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China
关键词：
wheat;intracultivaral chromosome substitution lines;agronomic traits;high molecular weight glutenin subunits (HMW-GS);molecular markers
摘  要：
Locating of important agronomic genes onto chromosome is helpful for efficient development of new wheat varieties. Wheat chromosome substitution lines between two varieties have been widely used for locating genes because of their distinctive advantages in genetic analysis, compared with the aneuploid genetic materials. Apart from the substituted chromosome, the other chromosomes between the substitution lines and their recipient parent should be identical, which eases the gene locating practice. In this study, a set of chromosome substitution lines with cv. Wichita (WI) as the recipient parent and cv. Cheyenne (CNN) as the donor parent were studied for the composition of high molecular weight glutenin subunits (HMW-GS) as well as a range of agronomic important traits. Results revealed that the substitution lines of WI(CNN5D), WI(CNN6A) and WI(CNN7B) had higher plant heights than the two parents of WI and CNN, and WI(CNN3D) had later maturity than the parents. By sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis, a substitution line WI(CNN5B) was found to contain different HMW-GS patterns from its two parents, in which 1By9 was replaced by 1By8 on chromosome 1BL. Simple sequence repeat (SSR) analysis confirmed that the variation on 1BL in WI(CNN5B) was originated from Chinese Spring (CS). It is concluded that chromosomal fragments from bridge material and donor parent were quite often retained in intracultivaral chromosome substitution lines except the substituting chromosomes.
作  者：
ZHAO Pei;WANG Ke;LIN Zhi-shan;LIU Hui-yun;LI Xin;DU Li-pu;YAN Yue-ming;YE Xing-guo;National Key Facility of Crop Gene Resources and Genetic Improvement,Institute of Crop Science, Chinese Academy of Agricultural Sciences;Key Laboratory of Genetics and Biotechnology, College of Life Science, Capital Normal University;
关键词：
wheat;;intracultivaral chromosome substitution lines;;agronomic traits;;high molecular weight glutenin subunits(HMW-GS);;molecular markers
摘  要：
Locating of important agronomic genes onto chromosome is helpful for efficient development of new wheat varieties. Wheat chromosome substitution lines between two varieties have been widely used for locating genes because of their distinctive advantages in genetic analysis, compared with the aneuploid genetic materials. Apart from the substituted chromosome, the other chromosomes between the substitution lines and their recipient parent should be identical, which eases the gene locating practice. In this study, a set of chromosome substitution lines with cv. Wichita(WI) as the recipient parent and cv. Cheyenne(CNN) as the donor parent were studied for the composition of high molecular weight glutenin subunits(HMW-GS) as well as a range of agronomic important traits. Results revealed that the substitution lines of WI(CNN5D), WI(CNN6A) and WI(CNN7B) had higher plant heights than the two parents of WI and CNN, and WI(CNN3D) had later maturity than the parents. By sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS) analysis, a substitution line WI(CNN5B) was found to contain different HMW-GS patterns from its two parents, in which 1By9 was replaced by 1By8 on chromosome 1BL. Simple sequence repeat(SSR) analysis confirmed that the variation on 1BL in WI(CNN5B) was originated from Chinese Spring(CS). It is concluded that chromosomal fragments from bridge material and donor parent were quite often retained in intracultivaral chromosome substitution lines except the substituting chromosomes.

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