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当前位置: 首页 > 文章 > HPLC法测定LED不同光质下迷迭香愈伤组织中鼠尾草酸含量 广东农业科学 2015,42 (15) 77-81+2
Position: Home > Articles > Carnosic acid content determination in Rosmarinus officinalis L. callus under different qualities of LED light by HPLC Guangdong Agricultural Sciences 2015,42 (15) 77-81+2

HPLC法测定LED不同光质下迷迭香愈伤组织中鼠尾草酸含量

作  者：
徐小萍;谢燕萍;慕尧;钟春水;陈芳兰;张梓浩;陈裕坤;郭容芳;林玉玲;赖钟雄
单  位：
福建农林大学园艺学院/园艺植物生物工程研究所
关键词：
迷迭香;愈伤组织;LED;光质;鼠尾草酸;HPLC
摘  要：
以迷迭香(Rosmarinus officinalis L.)愈伤组织为材料,采用高效液相色谱法(HPLC)测定了不同LED光质下迷迭香愈伤组织中鼠尾草酸的含量。HPLC法测定鼠尾草酸的色谱条件为:C18色谱柱,柱温30℃;流动相为乙腈∶0.1%磷酸=55∶45(V/V),流速1.5 ml/min,紫外检测波长210 nm,等度洗脱,时间30 min。结果表明:与黑暗和日光灯白光下的愈伤组织对照,LED黄光下愈伤组织生长最好,LED白光下生长较好,LED蓝光下生长最差;不同LED光质下迷迭香愈伤组织中鼠尾草酸含量从高到低依次为:LED白光>日光灯白光>LED蓝光>LED红光>LED绿光>LED黄光;LED白光下迷迭香愈伤组织鼠尾草酸含量最高,同时较利于愈伤组织生长。
译  名：
Carnosic acid content determination in Rosmarinus officinalis L. callus under different qualities of LED light by HPLC
作  者：
XU Xiao-ping;XIE Yan-ping;MU Yao;ZHONG Chun-shui;CHEN Fang-lan;ZHANG Zi-hao;CHEN Yu-kun;GUO Rong-fang;LIN Yu-ling;LAI Zhong-xiong;College of Horticulture,Fujian Agriculture and Forestry University/Institute of Horticultural Biotechnology;
关键词：
Rosmarinus officinalis L.;;callus;;LED;;light quality;;carnosic acid;;HPLC
摘  要：
The callus of rosemary(Rosmarinus officinalis L.)was used as materials,the content of carnosic acid in callus under different LED light was detected by High Performance Liquid Chromatography(HPLC). The C18 chromatographic column was utilized for analysis. The column was eluted at 30℃ with isocratic elution of 55% acetonitrile and 45% solution(0.1% phosphoric acid)for 30 min at a flow rate of 1.5 m L/min. The elunet was monitored at 280 nm by using UV spectra. The results showed that compared with callus under dark and white fluorescent lamp,the callus cultured under LED yellow grew best, those under LED white also grew well,while those under LED blue grew worst. The order of carnosic acid content of rosemary callus under different LED lights was LED white > white fluorescent lamp > LED blue > LED red > LED green > LED yellow. Under LED white,the content of carnosic acid in callus was the highest and the callus also grew well.

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