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当前位置: 首页 > 文章 > 苯胺高效降解菌的筛选及其生物学特性研究 农业环境科学学报 2007,26 (05) 1738-1743
Position: Home > Articles > Isolation and Characterization of Aniline-Degradation Bacterial Strains Journal of Agro-Environment Science 2007,26 (05) 1738-1743

苯胺高效降解菌的筛选及其生物学特性研究

作  者：
李岩;李社增;鹿秀云;刘光耀;蒋继志;马平
单  位：
河北省农林科学院植物保护研究所河北省农业有害生物综合防治工程技术研究中心;河北大学生命科学学院
关键词：
苯胺;降解;鉴定;假单胞菌
摘  要：
采用富集培养法从高阳印染厂排污口土壤中分离得到209株微生物,定向筛选获得2株能够高效降解苯胺的细菌(菌株Ani-4-15和菌株Ani-5-61)。这2株细菌在苯胺浓度为400mg·L-1的培养液中培养30h后,培养液中苯胺的降解率均可达到85%以上;在苯胺浓度为1000mg·L-1的培养液中培养30h后,培养液中苯胺的降解率达70%左右。通过浊度测定法对菌株Ani-4-15和Ani-5-61在苯胺选择性培养基中的生长特性进行了研究,结果表明,两菌株最佳培养时间分别为15h和18h,最适生长温度均为30℃,最适生长pH值分别为7.0和6.0,对苯胺的耐受浓度范围在100～3200mg·L-1之间。在温室条件下,通过在灭菌土中分别接入一定量的苯胺(苯胺含量分别为400、600、800和1000mg·kg-1)和苯胺降解菌(106个菌体·g-1土),48h时菌株Ani-4-15和Ani-5-61对苯胺的降解率分别高达93.4%和96.6%。通过16SrDNA序列分析法明确了两株细菌均为假单胞菌属,利用非肠道革兰氏阴性杆菌鉴定系统(API20NE)进一步鉴定到种,菌株Ani-4-15为恶臭假单胞菌(Pseudomonas putida),菌株Ani-5-61为施氏假单胞菌(Pseudomonas stutzeri)。
译  名：
Isolation and Characterization of Aniline-Degradation Bacterial Strains
作  者：
LI Yan1,2,LI She-zeng1,LU Xiu-yun1,LIU Guang-yao1,JIANG Ji-zhi2,MA Ping1 (1.Institute of Plant Protection,Hebei Academy of Agricultural and Forestry Sciences,IPM Centre of Hebei Province,Baoding 071000,China; 2.College of Life Science,Hebei University,Baoding 071002,China )
关键词：
aniline; bio-degradation; identification; Pseudomonas sp.
摘  要：
Two hundred and nine strains of microorganisms were isolated from the sewage soil of the printworks in Gaoyang county. Among them,two strains of aniline-degradation bacteria,Ani-4-15 and Ani-5-61 that could use aniline as sole carbon and nitrogen sources,were selected. Aniline degradation rates by the two strains were all over 85% when the strains were inoculated in liquid medium with 400 mg·L-1 aniline for 30 h,and the aniline degradation rates were all around 70% in liquid medium with 1 000 mg·L-1 aniline for 30 h. Aniline-degradation ability of the strains was investigated in the greenhouse condition. The result showed that 93.4% and 96.6% aniline were degraded by strains Ani-4-15 and Ani-5-61,respectively. Preliminary study on growth of the two strains was tested in the selected broth. The optimum culture time of the two strains was 15 h and 18 h,the optimum growth temperature was 30 ℃,and the optimum pH values of Ani-4-15 and Ani-5-61 were 7.0 and 6.0,respectively. Strain Ani-4-15 was Pseudomonas putida and strain Ani-5-61 was P. stutzeri based on the identification through 16S rDNA ITS,biological characteristics and API 20 NE system.

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