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Position: Home > Articles > Establishment of liquiChip for detection of medroxyprogesterone acetate residue Animal Husbandry & Veterinary Medicine 2012,44 (9) 14-19

醋酸甲羟孕酮的液相芯片法检测体系建立

作  者:
陈政晓;王艳;李健;陈沁
单  位:
上海出入境检验检疫局;上海大学
关键词:
液相芯片;醋酸甲羟孕酮;残留;检测
摘  要:
本研究通过抗原抗体的浓度、藻红蛋白标记的链霉亲和素(SAPE)浓度等试验条件的优化,建立了醋酸甲羟孕酮的液相芯片检测体系,并对抗体的稳定性及特异性进行了分析。结果表明,500个微球、SAPE稀释度为1∶100、抗体浓度1∶3 000时,醋酸甲羟孕酮的标准曲线为:FI=26.7208+6880.2792/((1+(X/3.69262)0.958863))1.27862,曲线关系良好,该方法检测限为0.429 ng/mL,检测过程约需2 h。利用该方法检测样品,结果偏差较小,说明该方法有一定的实用价值。
译  名:
Establishment of liquiChip for detection of medroxyprogesterone acetate residue
作  者:
CHEN Zheng-xiao1,WANG Yan2,LI Jian2,CHEN Qin1(1.Shanghai University,Shanghai 200444,China; 2.Shanghai Entry-Exit Inspection and Quarantine,Shanghai 200135,China)
关键词:
LiquiChip technique;medroxyprogesterone acetate;residue;detection
摘  要:
On the basis of the optimization of the experimental conditions such as the concentrations of antigen,antibody and PE-streptavidin(SAPE),a rapid and sensitive method for the detection of medroxyprogesterone acetate residue based on the multiplexed Luminex assay was established.At the same time the stability and specificity of the detection antibody were analyzed.The results showed that the standard curve was FI=26.7208+6880.2792/((1+(X/3.69262)∧0.958863))∧1.27862 when 500 beads were used with 1∶100 of SAPE and 1∶3000 of biotin.The detection limit was 0.429ng/mL.This process usually takes 2 hours.The result indicated that Luminex assay would have a great potential in clinical application.

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