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当前位置: 首页 > 文章 > 高良姜多糖提取工艺优化及其抗氧化活性 食品科学 2014,35 (2) 126-131
Position: Home > Articles > Optimization of Extraction Process and Antioxidant Activities of Polysaccharides from Alpinia officinarum Hance FOOD SCIENCE 2014,35 (2) 126-131

高良姜多糖提取工艺优化及其抗氧化活性

作  者：
郑义;王卫东;李勇;朱园园;郭静
单  位：
徐州工程学院食品(生物)工程学院
关键词：
高良姜;多糖;响应面法;抗氧化活性
摘  要：
通过Box-Behnken试验设计,获得了热水浸提高良姜多糖的最佳工艺;以清除1,1-二苯基-2-三硝基苯肼(DPPH)自由基能力、还原力、清除羟自由基能力、螯合铁离子能力为指标,评价了高良姜多糖的抗氧化活性。结果表明,热水浸提高良姜多糖的最佳工艺条件为液料比43∶1(mL/g)、浸提温度95℃、浸提时间3 h,在此条件下多糖得率实测值为11.81%。高良姜多糖具有较好的抗氧化活性,清除自由基能力、还原力和螯合铁离子能力均表现出一定的质量浓度依赖性;高良姜多糖清除DPPH自由基、清除羟自由基和螯合铁离子能力的半数有效质量浓度(EC50)分别为(0.59±0.01)、(0.05±0.003)g/L和(2.75±0.2)g/L。
译  名：
Optimization of Extraction Process and Antioxidant Activities of Polysaccharides from Alpinia officinarum Hance
作  者：
ZHENG Yi;WANG Wei-dong;LI Yong;ZHU Yuan-yuan;GUO Jing;College of Food (Biology) Engineering,Xuzhou Institute of Technology;Jiangsu Key Construction Laboratory of Food Resource Development and Quality Safe;
关键词：
Alpinia officinarum Hance;;polysaccharides;;response surface methodology;;antioxidant activity
摘  要：
The conditions for hot water extraction of polysaccharides from Alpinia officinarum Hance were optimized by Box-Behnken statistical design. The antioxidant activities of polysaccharides extracted from Alpinia officinarum Hance were investigated using four different in vitro antioxidant assays, 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical scavenging activity, reducing power, hydroxyl radical scavenging activity and iron-chelating ability. The results showed that the optimal extraction conditions were found to be extraction at 95 ℃ for 3 h with a solvent-to-solid ratio of 43:1(mL/g). The experimentally observed yield of polysaccharides extracted from Alpinia officinarum Hance was 11.81% under these conditions. The extracted polysaccharides had powerful antioxidant activities for free radical scavenging activity, reducing power and ironchelating capacity in concentration-dependent manner, with median effective concentration(EC50) of(0.59 ± 0.01),(0.05 ± 0.003) and(2.75 ± 0.2) g/L, respectively.

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