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两种方法检测奶牛结核病的比较分析

作  者:
王丽霞;李爱华;王会杰;刘翠梅;刘天驹;李翀;韩庆安
单  位:
秦皇岛市畜牧站河北秦皇岛066001;河北省动物疫病预防控制中心河北保定071000;盐山县动物疫病预防控制中心河北沧州061300;秦皇岛市动物疫病预防控制中心河北秦皇岛066001;中共迁安市委农工委河北迁安064400
关键词:
奶牛结核病;皮内变态反应;IFN-γ试验
摘  要:
采用常规皮内变态反应(TST方法)对唐山、秦皇岛、沧州和石家庄四个市共计2 204头奶牛进行牛结核病检测,采用IFN-γ试验方法对PPD检测结果为阳性、可疑的牛以及部分阴性牛进行复检。将IFN-γ试验与TST检测结果相比较,其敏感性为78.26%,特异性为89.09%,符合率为84.33%。研究表明单独使用PPD皮内变态反应方法在牛结核病的诊断方面存在特异性差的缺点,用IFN-γ试验对TST试验阳性和可疑牛进行复检可以提高特异性,避免造成假阳性牛的误杀;该方法在临床上适合推广应用。
译  名:
Comparison of Two Methods for Detection of Bovine Tuberculosis
作  者:
Wang Lixia;Li Aihua;Wang Huijie;Liu Cuimei;Liu Tianju;Li Chong;Han Qingan;Rural Work Community of Qian'an City;Animal Disease Prevention and Control Center of Qin Huangdao City;Animal Husbandry and Veterinary Station of Qin Huangdao City;Animal Disease Prevention and Control Center of Yanshan County;Animal Disease Prevention and Control Center of Hebei Province;
关键词:
bovine tuberculosis;;tuberculin skin test;;γ-interferon assay
摘  要:
A total of 2204 cows from Tangshan, Qin Huangdao, Cangzhou and Shi Jiazhuang were checked for bovine tuberculosis with the method of tuberculin skin test(TST), of which the positive, doubtful and some negative results were rechecked by γ- interferon assay. The results of γ- interferon assay which has a sensitivity of 78.26% and a specificity of 84.33% were compared to those of TST. According to that, the consistency rate of the two methods reached 84.33%. The study showed that γ- interferon assay, which could improve the specificity and therefore avoid false culling, was suited for widespread clinical use.

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