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两种奶牛结核病检测方法的比较

作  者:
徐贤坤;黄小武;蓝纪;黄胜斌;熊毅;刘棋;韦正吉;黄益泓
单  位:
广西柳州市动物疫病预防控制中心;广西南宁市动物疫病预防控制中心;广西动物疫病预防控制中心
关键词:
奶牛结核病;皮内变态反应;抗原特异性IFN-γ试验
摘  要:
本研究中采用常规皮内变态反应(TST)对广西南宁、柳州共计2 818头黑白花奶牛进行牛结核病检测,采用抗原特异性IFN-γ试验方法对PPD检测结果为阳性、可疑的牛以及部分阴性牛进行复检。将抗原特异性IFN-γ试验与TST检测结果相比,其敏感性为53.33%,特异性为70.49%,符合率为67.11%。本研究证实单独使用PPD皮内变态反应方法在牛结核病的诊断方面存在非特异性强等缺点,易造成假阳性牛的误杀;用抗原特异性IFN-γ试验对TST试验阳性和可疑牛进行复检可以提高特异性,该方法在临床上有重要应用价值。
译  名:
Comparison of two methods in the diagnosis of bovine tuberculosis
作  者:
XU Xian-kun1,HUANG Xiao-wu2,LAN Ji3,HUANG Sheng-bin1,XIONG Yi1,LIU Qi1,WEI Zheng-ji2,HUANG Yi-hong2(1.Guangxi center for Animal Disease Prevention and Control,Nanning 530001,China;2.Liuzhou center for Animal Disease Prevention and Control,Liuzhou 545005,China;3.Nanning center for Animal Disease Prevention and Control,Nanning 530001,China)
关键词:
bovine tuberculosis;tuberculin skin test;IFN-γassay
摘  要:
2818 cattle were detected for Mycobacterium tuberculosis by tuberculin skin test(TST),and the interferon gamma(IFN-γ) assay was used to retest the positive,suspicious and some negative individuals.Compared with TST,the diagnosis sensitivity,specificity and coincidence of IFN-γ assay were 53.33%,70.49% and 67.11%,respectively.This study demonstrated that the use of TST alone in the diagnosis of bovine tuberculosis has non-specific defects and the retest with antigen-specific IFN-γ assay for the positive and suspicious individuals by TST can improve the specificity.The IFN-γ assay would be a useful tool in the diagnosis of bovine tuberculosis.

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