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Position: Home > Articles > Antioxidant Activities in vitro and in Cells and Amino Acid Composition of Rapeseed Protein Hydrolysates(RPHs) FOOD SCIENCE 2014,35 (13) 49-53

菜籽蛋白水解物体外和细胞内抗氧化性评价及氨基酸分析研究

作  者:
王立峰;王玉梅;张晶;谢慧慧;鞠兴荣
单  位:
南京财经大学食品科学与工程学院
关键词:
菜籽肽;抗氧化能力指数;细胞抗氧化活性;氨基酸分析;电泳
摘  要:
以"双低"油菜籽秦优7号为原料,碱溶酸沉法获得菜籽分离蛋白后进一步用Alcalase2.4L酶解得到菜籽蛋白水解物(rapeseed protein hydrolysates,RPHs);采用聚丙烯酰胺葡聚糖凝胶柱(Sephacryl S-100HR)对RPHs进行分离纯化,得到3个组分;采用抗氧化能力指数(oxygen radical absortion capacity,ORAC)方法以及细胞抗氧化活性(cellular antioxidant capacity,CAA)的方法分析筛选得到抗氧化性最好的组分3。结果表明组分3的ORAC值为(1610.38±112.51)μmol TE/g;CAA值为(124.66±2.18)μmol QE/g,EC50值为(57.84±3.38)μg/mL;在此基础之上,对组分3进行氨基酸分析以及电泳分析,表明其抗氧化性与分子质量分布及氨基酸组成可能存在一定关系。
译  名:
Antioxidant Activities in vitro and in Cells and Amino Acid Composition of Rapeseed Protein Hydrolysates(RPHs)
作  者:
WANG Li-feng;WANG Yu-mei;ZHANG Jing;XIE Hui-hui;JU Xing-rong;School of Food Science and Engineering,Nanjing University of Finances and Economics;
关键词:
rapeseed protein hydrolysates;;oxygen radical absortion capacity(ORAC);;cellular antioxidant capacity(CAA);;amino acid analysis;;electrophoresis
摘  要:
In this study, rapeseed proteins obtained from defatted meal of double-zero rapeseed(Qinyou No. 7) by alkaline extraction and acid precipitation were hydrolyzed with Alcalase 2.4L to produce rapeseed protein hydrolysates(RPHs). After chromatographic separation of RPHs on a Sephacryl S-100 HR column, three fractions were harvested. Fraction III showed the most potent antioxidant activity in terms of oxygen radical absortion capacity(ORAC) and cellular antioxidant capacity(CAA). This fraction had an ORAC value of(1 610.38 ± 112.51) μmol TE/g, a CAA value of(124.66 ± 2.18) μmol QE/g, and an EC50 value of(57.84 ± 3.38) μg/mL. Furthermore, the results of amino acid composition analysis and electrophoresis indicated a possible association between the antioxidant activities and either molecular weight distribution or amino acid composition.

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