作 者：
杨竹鸣;陈善真;李其昌;刘小琴;王贵平;范红结;李中圣
关键词：
PLL;PIFNα;猪繁殖和呼吸综合征;中和抗体;IFN-γ
摘 要：
本文探讨聚合体形式的猪干扰素α真核表达质粒(p VAX1-PIFNα)对猪PRRSV弱毒疫苗免疫作用的增强作用。在猪免疫PRRSV弱毒疫苗的同时,注射p VAX1-PIFNα重组表达质粒与多聚赖氨酸(PLL)的聚合体(PLL-p VAX1-PIFNα),以研究聚合体状态的干扰素对PRRSV免疫的增强作用。将分泌表达干扰素α的重组真核表达质粒p VAX1-PIFNα与PLL聚合,同时免疫PRRSV弱毒疫苗和PLL-p VAX1-PIFNα聚合体,使用ELISA试剂盒检测PRRSV中和抗体;另外,分离培养猪外周血白细胞(PBMC)进行体外试验,分析PLL-p VAX1-PIFNα聚合体刺激淋巴细胞后IFN-γ的分泌情况。中和抗体的结果表明,PLL-p VAX1-PIFNα的使用可明显提高PRRSV活疫苗诱导产生PRRSV中和抗体的能力;细胞试验的结果表明,经PLL-p VAX1-PIFNα刺激后,明显提高了淋巴细胞分泌表达IFN-γ的水平。在PRRSV疫苗免疫中,聚合体状态的猪IFNα重组表达质粒可有效提高机体的体液免疫和细胞免疫水平,这一结果也为干扰素或其他重组蛋白在疫苗免疫中的应用提供了新的思路。
译 名：
Study of PLL-p VAX1-PIFNα polymer in PRRSV vaccine immunity enhancement
作 者：
YANG Zhu-ming;CHEN Shan-zhen;LI Qi-chang;LIU Xiao-qin;WANG Gui-ping;FAN Hong-jie;LI Zhong-sheng;Guangdong Haid Institute of Animal Husbandry & Veterinary;Nanjing Agricultural University;
关键词：
PLL;;PIFNα;;PRRS;;Neutralizing antibody;;IFN-γ
摘 要：
To evaluate the effect of PLL-p VAX1-PIFNα on PRRSV vaccine immunity,piglets were injected with both PRRSV attenuated vaccine and PLL-p VAX1-PIFNα in the neck. In this study,the plasmid of p VAX1-IFNα was polymerized with PLL firstly,the polyme were then administered with PRRSV attenuated vaccine in the neck. The serum was collected at different time-posts injection,and the neutralization antibody of PRRSV was tested with ELISA kit. In the in vitro test,the peripheral blood was acquired from each pig firstly,then the T lymphocytes were isolated and stimulated with PLL-p VAX1-PIFNα,the cell culture medium was collected at different time-posts inoculation,and the IFN-γ were tested with ELISA kit. In the result,the level of neutralization antibody of PRRSV in the PLL-p VAX1-PIFNα injected group was significantly higher than the control. From the results of in vitro test,the IFN-γ polymer was unregulated.It concluded that The polymer,PLL-p VAX1-PIFNα,could effectively improve the humoral immunity and cellular immunity.This result also pave a new way for the use of IFNα and other proteins in vaccine immunity.
