作 者：
赵天勤;杜杰;金春阳;操风;潘彦鹏;曹献英
摘 要：
以还原胺化反应制得PLL-S共聚物,并通过红外光谱以及核磁共振对其进行了结构表征,采用凝胶渗透色谱法检测了PLL-S的相对分子质量,激光粒度分析仪检测了其电位,琼脂糖凝胶电泳法研究了其结合DNA的能力及抗DNaseⅠ的能力,MTT法分析了PLL修饰前后的细胞毒性,并研究了其在293T细胞中转染质粒DNA的能力。结果表明:制备的PLL-S共聚物接枝率为2.8%,数均相对分子质量为60629,电位为23.7mV,达到细胞毒性评价等级0级和1级,材料是合格的,可结合DNA且能保护DNA免受DNaseⅠ的破坏,在293T细胞中的转染效率达到了(48±3.2)%。经对PLL-S的性能分析可以判断:该共聚物可作为基因载体在生物医学方面予以应用。
译 名：
Research on the Preparation and Property of PLL-grafted Starch Copolymer as Gene Carriers
作 者：
ZHAO Tianqin,DU Jie,JIN Chunyang,CAO Feng,PAN Yanpeng,CAO Xianying ( College of Material and Engineer,Hainan University,Haikou 570228,China)
关键词：
PLL; soluble starch; gene carrier
摘 要：
Poly-L-lysine-Starch ( PLL-S) copolymer was prepared by reductive amination reaction. The PLL-S copolymer were characterized by infrared spectroscopy and nuclear magnetic resonance spectroscopy,and the molecular weights of the PLL-S were examined by gel chromatography,with a laser particle size analyzer to detect its potential. We examined the ability of the PLL to DNA-binding and degradation of the ability of anti-DNase Ⅰ using agarose gel electrophoresis. The cytotoxicity of PLL decorated with starch modified was analyzed through MTT method in 293T cells. We assayed the PLL-S transfecting plasmid DNA of capability. The results showed that the prepared PLL-S graft ratio was 2. 8% ,a number average molecular weight was 60 629,potentials was 23. 7 mV. PLL-S copolymer was capable of binding to DNA,and protected DNA from the destruction of the DNase Ⅰ. The cytotoxicity of the copolymer was low enough to achieve evaluation level of one or more standard. transfection efficiency of the PLL-S copolymer was as high as ( 48 ± 3. 2) % . We judged that PLL-S copolymer prepared can be used as gene carriers in biomedical application by studying its performance.
