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Position: Home > Articles > PCR Amplification and Cloning of the Genomic FragmentEncoding Helianthus tuberosus Agglutinin Journal of Henan Agricultural Sciences 2004,33 (3) 11-14

菊芋凝集素基因组片段的PCR扩增及克隆

作  者:
刘茵;刘秀花;常团结
单  位:
商丘师范学院生物系
关键词:
菊芋;凝集素;基因组片段;聚合酶链式反应(polymerase chain reaction,PCR)
摘  要:
通过PCR扩增 ,获得了菊芋凝集素基因 (hta)的编码区基因组片段hta - g。DNA序列分析表明 ,PCR产物长 70 2bp ,包括 444bp的编码区序列 ,编码区被一个 2 5 8bp的内含子分成 2个长度分别为 2 1 0bp及 2 34bp外显子 ,内含子边界序列符合GT/AG规则。与htacD NA序列的比较分析表明 ,hta - g的编码区序列与htacDNA序列高度同源 ,同源性在 93%~98%。
译  名:
PCR Amplification and Cloning of the Genomic FragmentEncoding Helianthus tuberosus Agglutinin
作  者:
LIU Yin,LIU Xiu-hua,CHANG Tuan-jie(Department of Biology, Shangqiu Normal College,Shangqiu 476000,China)
关键词:
Helianthus tuberosus L;Agglutinin;Genomic fragment;PCR
摘  要:
The genomic sequence hta-g covering the coding region of hta was amplified by PCR and cloned using pGEMT-easy vector.DNA sequence analysis showed that the PCR production is 702 bp in longth with the coding region of 444 bp.The coding region is divided into two exons of 210 bp and 234 bp by an intron of 258 bp.The dinucleotides flanking the introns conform to the GT/AG rule for splice junctions. The coding sequence of hta-g is in high homologies with the hta cDNA sequences in a range from 93% to 98%.

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