中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > The Effect of Administration of Rutin on Plasma Levels of Estrogen, Prolactin, Growth Hormone and Gene Expression of Their Receptors in Mammary Glands in Ovariectomized Rats 农业科学学报 (英文) 2012,11 (10)
Position: Home > Articles > The Effect of Administration of Rutin on Plasma Levels of Estrogen, Prolactin, Growth Hormone and Gene Expression of Their Receptors in Mammary Glands in Ovariectomized Rats Journal of Integrative Agriculture 2012,11 (10)

The Effect of Administration of Rutin on Plasma Levels of Estrogen, Prolactin, Growth Hormone and Gene Expression of Their Receptors in Mammary Glands in Ovariectomized Rats

作  者：
Guo Xu-dong;Diao Qi-yu;Wang Yue-ying;Tu Yan;Deng Kai-dong;Wang Xin-jian;Fu Tong;Yan Gui-long
单  位：
Henan Agr Univ, Key Lab Anim Growth & Dev, Minist Agr, Zhengzhou 450002, Peoples R China;Jinling Inst Technol, Coll Anim Sci, Nanjing 210038, Jiangsu, Peoples R China;Chinese Acad Agr Sci, Feed Res Inst, Minist Agr, Key Lab Feed Biotechnol, Beijing 100081, Peoples R China;Hebei North Univ, Coll Anim Sci, Zhangjiakou 075000, Peoples R China
关键词：
rutin;rats;mammary gland development;hormones;receptors
摘  要：
The development of mammary glands, endocrine hormone concentrations and the gene expression of related receptors were measured in ovariectomized virgin rats after adminstration of an estrogen-like plant extract, rutin. Thirty-two ovariectomized virgin Wistar rats were randomly assigned to 4 treatments with 8 animals each: gastric infusion of 2 mL normal saline per unovariectomized rat per day (Sham), gastric infusion of 2 mL normal saline per ovariectomized rat per day (Ova), gastric infusion of 60 mg rutin kg(-1) body weight (BW) per ovariectomized rat per day (Ova+Rut), or intramuscular injection of 60 mu g estradiol kg(-1) BW per ovariectomized rat weekly (Ova+Est). Samples of blood and mammary glands were harvested to determine the levels of estrogen (E-2), prolactin (PRL) and growth hormone (GH), and the gene expression of estrogen receptors (ER), prolactin receptors (PRLR) and growth hormone receptors (GHR) with radioimmunoassy (RIA) and RT-PCR technology, respectively. The E-2 concentration in plasma and gland tissues from the rats of Ovx+Rut or Ovx+Est was higher than that of Ovx (P<0.05), but the plasma E-2 concentration from the rats of Ovx+Rut was lower than that of Sham (P<0.05). The order of the PRL concentration in plasma and gland tissues was Ovx
作  者：
GUO Xu-dong1, DIAO Qi-yu1, WANG Yue-ying2, TU Yan1, DENG Kai-dong3, WANG Xin-jian2, FU Tong2 and YAN Gui-long4 1 Key Laboratory of Feed Biotechnology, Ministry of Agriculture/Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China 2 Key Laboratory of Animal Growth and Development, Ministry of Agriculture/Henan Agricultual University, Zhengzhou 450002, P.R. China 3 College of Animal Sciences, Jinling Institute of Technology, Nanjing 210038, P.R.China 4 College of Animal Sciences, Hebei North University, Zhangjiakou 075000, P.R.China
关键词：
rutin, rats, mammary gland development, hormones, receptors
摘  要：
The development of mammary glands, endocrine hormone concentrations and the gene expression of related receptors were measured in ovariectomized virgin rats after adminstration of an estrogen-like plant extract, rutin. Thirty-two ovariectomized virgin Wistar rats were randomly assigned to 4 treatments with 8 animals each: gastric infusion of 2mL normal saline per unovariectomized rat per day (Sham), gastric infusion of 2mL normal saline per ovariectomized rat per day (Ova), gastric infusion of 60mg rutin kg-1 body weight (BW) per ovariectomized rat per day (Ova+Rut), or intramuscular injection of 60μg estradiol kg-1 BW per ovariectomized rat weekly (Ova+Est). Samples of blood and mammary glands were harvested to determine the levels of estrogen (E2 ), prolactin (PRL) and growth hormone (GH), and the gene expression of estrogen receptors (ER), prolactin receptors (PRLR) and growth hormone receptors (GHR) with radioimmunoassy (RIA) and RT-PCR technology, respectively. The E2 concentration in plasma and gland tissues from the rats of Ovx+Rut or Ovx+Est was higher than that of Ovx (P<0.05), but the plasma E2 concentration from the rats of Ovx+Rut was lower than that of Sham (P<0.05). The order of the PRL concentration in plasma and gland tissues was Ovx

相似文章

导出引用
计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46