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当前位置: 首页 > 文章 > 紫锥菊根中菊苣酸的提取分离及其分子印迹聚合物的制备 动物营养学报 2019 (8) 3914-3920
Position: Home > Articles > 紫锥菊根中菊苣酸的提取分离及其分子印迹聚合物的制备 Chinese Journal of Animal Nutrition 2019 (8) 3914-3920

紫锥菊根中菊苣酸的提取分离及其分子印迹聚合物的制备

作  者：
张虎;王庚南;王建平;马雨红;苏铭;刘聚祥
单  位：
河北农业大学
关键词：
菊苣酸;分子印迹聚合物;提取;纯化;高效液相色谱
摘  要：
菊苣酸为天然药物紫锥菊中有效酚酸类化合物,本试验旨在研究紫锥菊根中菊苣酸提取纯化的新方法.试验采用超声辅助提取法对紫锥菊根粉末进行提取,所得粗提物再用葡聚糖凝胶LH-20为固定相,80%、60%及40%甲醇为流动相,进行反复提取,得到菊苣酸单体.再将制得的菊苣酸单体作为模版分子,α-甲基丙烯酸为功能单体,乙二醇二甲基烯酸酯为交联剂,制备菊苣酸分子印迹聚合物,并应用此分子印迹聚合物为固定相,对紫锥菊根粉进行富集纯化.结果显示,经葡聚糖凝胶LH-20提取分离后,高效液相色谱法测定菊苣酸含量为90.22%.紫锥菊根粉经分子印迹聚合物富集纯化后,可同时富集母核结构相同的菊苣酸和咖啡酸.由此可见,本方法可用于紫锥菊根中菊苣酸的提取分离及其分子印迹聚合物的制备.
作  者：
ZHANG Hu;WANG Gengnan;WANG Jianping;MA Yuhong;SU Ming;LIU Juxiang;College of Veterinary Medicine,Agricultural University of Hebei;
关键词：
chicoric acid;;molecularly imprinted polymer;;extraction;;purification;;HPLC
摘  要：
Chicoric acid is one active phenolic acid component of natural herbal medicine Echinacea purpurea,and this study set out to investigate the new isolation and purification method of chicoric acid from Echinacea purpurea root.The chicoric acid monomer was isolated and purified by the sephadex gel LH-20 column which eluted by 80%,60%,40% methanol after extracted by ultrasonic extraction from Echinacea purpurea root powder.For the preparation of molecularly imprinted polymer of chicoric acid, the purified chicoric acid monomer above was used as the template molecule with the 4-vinylpyridine and ethylene glycol dimethacrylate to be functional monomer and crosslinker,respectively,and the Echinacea purpurea root powder was enrichment purified by the molecularly imprinted polymer.The results showed that after sephadex gel LH-20 column separated and purified,the chioric acid content was 90.22% by high performance liquid chromatography.After enrichment purified by the molecularly imprinted polymer of Echinacea purpurea root powder,the cichoric acid and caffeic acid could simultaneous enrichment which was with the same mother nucleus structure.In conclusion,this method can used for the isolation and purification of chicoric acid from echinacea purpurea root and its molecularly imprinted polymer preparation.[Chinese Journal of Animal Nutrition,2019,31(8):3914-3920]

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