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当前位置: 首页 > 文章 > 紫锥菊提取工艺研究及有效成分含量测定 中兽医医药杂志 2015 (3) 54-56
Position: Home > Articles > Content determination and extraction process of effective component in Echinacea purpurea Journal of Traditional Chinese Veterinary Medicine 2015 (3) 54-56

紫锥菊提取工艺研究及有效成分含量测定

作  者：
伏健;史若男;李婉;刘水平;宋丽婷;刘进辉
单  位：
湖南农业大学动物医学院
关键词：
紫锥菊;菊苣酸;提取工艺;高效液相色谱法
摘  要：
探索紫锥菊的乙醇法最佳提取工艺,并对菊苣酸含量进行测定。试验选取紫锥菊头状花序,用乙醇进行提取,采用L9(34)正交试验、以有效成分菊苣酸的含量为指标优选工艺参数。结果表明,紫锥菊的乙醇法最佳提取工艺条件为:料液比15倍量、乙醇浓度60%、提取温度90℃、提取1 h。以菊苣酸标准品为对照,以高效液相检测菊苣酸含量,色谱柱:Phenomenex C18110A(250 mm×4.6 mm,5μm),流动相:乙腈-0.1%磷酸水溶液(38∶62),流速:1.00 m L/min,紫外波长:326 nm,HPLC测定纯度为48.41%。此法提取工艺稳定、可行,测定方法简便准确,重复性好,可用于紫锥菊中菊苣酸的质量控制。
译  名：
Content determination and extraction process of effective component in Echinacea purpurea
作  者：
FU Jian;SHI Ruo-nan;LI Wan;LIU Shui-ping;SONG Li-ting;LIU Jin-hui;College of Veterinary Medicine, Hunan Agricultural University;
关键词：
Echinacea purpurea;;cichoric acid;;extraction process;;HPLC
摘  要：
Objective: To explore the optimal ethanol extraction technology and determine the content of cichoric acid from Echinacea purpurea. Methods: Cichoric acid was extracted from Echinacea purpurea lead inflorescence site using ethanol and its content was determined by HPLC. The column of Phenomenex C18110A(250 mm×4.6 mm, 5 μm) was adopted with mixture of acetonitrile-0.1% phosphoric acid(38∶62) as mobile phase at flow rate of 1.00 m L/min, and the UV detector was set at 326 nm. The extraction process was optimized by L9(34) orthogonal design with cichoric acid as standard for quality control. Results:The best extraction condition obtained were as followed: the ratio of material to solvent was 1 ∶15, extracting for 1 hour each time by 60% of alcohol and 48.41% of purity was achieved by HPLC. Conclusion: This optimal technology is stable and workable. The determination method is simple, accurate and highly reproducible, so it can be used to control quality of cichoric acid in Echinacea purpurea.

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