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西伯利亚百合子房的组织培养及离体快繁研究

作  者:
申玉华;段永平;唐立红;黄园;李超
单  位:
赤峰学院生命科学系
关键词:
西伯利亚百合;组织培养;子房
摘  要:
以子房为外植体,对西伯利亚百合离体快繁进行研究。结果表明:试验浓度范围内适合不定芽诱导的培养基以MS+6-BA 1.0 mg/L+NAA 1.5 mg/L为佳,芽增殖最佳培养基为MS+6-BA 1.0 mg/L+NAA 0.2 mg/L,适合生根的培养基为MS0,生根率达100%,且根系发达,长势粗壮,练苗3~5 d后移栽,长势良好,移栽成活率达90%以上。
译  名:
In Vitro Ovary Culture and Rapid Propagation of Siberia
作  者:
SHEN Yu-hua,DUAN Yong-hua,TANG Li-hong,HUANG Yuan,LI Chao (Department of Life Science,Chifeng College,Chifeng,Inner Mongolia 024000,China)
关键词:
Siberia;Tissue culture;Ovary
摘  要:
Rapid propagation of Siberia was preliminary studied using ovaries for in vitro culture.Result showed that the optimum mediums for shoot induction,shoot proliferation and rooting were MS+6-BA 1.0 mg/L+NAA 1.5 mg/L,MS+6-BA 1.0 mg/L+NAA 0.2 mg/ L and MS0 respectively.Rooting rate could reach 100% and its root system was well developed,after acclimatization for 3~5 days,the bulblets were transplanted and grew well,survival rate after transplanting was above 90%.

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