Analysis of the population structure of endogenous yeast of Ischiodon scutellaris Fabricius in Xinjiang using high throughput sequencing combined with culturing technologies

MI Tao-Tao;FENG Li;WANG Chong;SUN Yan-Fei;XIONG Jie;LEI Yong-Hui;College of Life Sciences, Shihezi University;Xinjiang Entry-exit Inspection and Quarantine Bureau;Agricultural College of Shihezi University;

Ischiodon scutellaris Fabricius;;endogenous yeast;;high-throughput sequencing;;diversity analysis;;26S rDNA

To clear and definite the dominant endogenous yeast of Ischiodon scutellaris Fabricius and their distribution in Shihezi, Xinjiang, the diversity of yeast isolated from I.scutellaris were identified and analysed by culture-dependent and culture-independent. It could provided the utilization of yeast resources with scientific basis. By Illumina(MiSeq) high-throughput sequencing and bioinformatics analysis platform, as well as to the I.scutellaris filtrate enrichment culture, selecting the representative strains on sugar fermentation, carbon source utilization of physiological and biochemical tests and 26 s rDNA D1/D2 section sequencing of I.scutellaris in the available to train separation of yeast identification, analysis of system evolution, yeast species distribution in I.scutellaris and their microbial community structure were discovered. A total of 14 culture-dependent yeast strains were isolated from I.scutellaris, including Kodamaea, Saccharomyces, Wickerhamomyces. The ratio over 1.49% among 16 culture-independent yeast genus are as follows: Filobasidium, Udeniomyces, Candida, Metschnikowia, Pichia, Protocheca, Papiliotrema, Dipodascus, Kwoniella, Schizosaccharomyces, Acaromyces, Cryptococcus, Cystofilobasidium, Tetrapisispora, Aureobasidium, Zygosaccharomyces, etc. The results showed that endogenous yeasts isolated from Ischiodon scutellaris Fabricius were abundant and various. It contained abundant yeast populations. Therefore, more systemic researches on endogenous yeast populations isolated from insects were necessary.