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当前位置: 首页 > 文章 > 昆虫肠道蛋白酶作用下δ-内毒素的毒性肽及毒力特异性的变化 昆虫学报 1993 (3) 263-271
Position: Home > Articles > HOST SPECIFICITY OF BACILLUS THURINGIENSIS 5-ENDOTOX1N PROTEOLYSED BY PROTEASES OF LARVAL GUT JUICE Acta Entomologica Sinica 1993 (3) 263-271

昆虫肠道蛋白酶作用下δ-内毒素的毒性肽及毒力特异性的变化

作  者：
李荣森;盛竹莓
单  位：
中国科学院武汉病毒研究所
关键词：
苏云金芽孢杆菌;δ-内毒素;毒性多肽;鞘翅目
摘  要：
本文报道杀斜纹夜蛾(Prodenia litura)δ-内毒素和杀鞘翅目昆虫δ-内毒素经昆虫肠液蛋白酶及胰蛋白酶作用后,其毒性肽及毒力特异性的变化。以Sephadex G75柱层析提纯的130kD原毒素,经斜纹夜蛾幼虫肠液蛋白酶作用后,产生的70kD与75kD抗蛋白酶多肽(PRP)对斜纹夜蛾和家蚕皆有毒;经家蚕幼虫肠液蛋白酶作用后,产生的62kD与65kD的PRP失去对斜纹夜蛾的毒性,仅对家蚕有毒;经胰蛋白酶作用后产生的65kD与68kD的PRP,其毒力特性与经家蚕肠液蛋白酶作用后的相似。斜纹夜蛾肠液蛋白酶作用后产生的PRP,可进一步被家蚕肠液蛋白酶或胰蛋白酶降解为63—65kD的PRP,此种多肽对斜纹夜蛾无毒,对家蚕有毒。杀鞘翅目昆虫的原毒素不能为胰蛋白酶和粘虫肠液所完全降解。证明不同种类昆虫的肠道蛋白酶对δ-内毒素蛋白质的作用位点不同,δ-内毒素对宿主昆虫的毒力特异性与其肠道蛋白酶的特性密切相关。
译  名：
HOST SPECIFICITY OF BACILLUS THURINGIENSIS 5-ENDOTOX1N PROTEOLYSED BY PROTEASES OF LARVAL GUT JUICE
作  者：
LI RONG-SEN SHENG ZHU-MEI(Wuhan Institute of Virology, Academia Simca, Wuhan 430071)
关键词：
Bacillus thuringiensis- σ-endotoxin-toxic polypetide-Coleoptera
摘  要：
Noctuids-sensitive parasporal crystals were dissolved and protoxins were activated with trypsin or gut juice of larva of Prodenia litura and Bombyx mori. Toxic polypeptide produced by digestion of B. mori gut juice or trypsin was toxic only to B. mori, whereas toxic polypeptide pro-duced by gut juice of P. litura was toxic to both P. litura and B. mori. Coleoptera-sensitive endotoxins of 70 kD and 50kD could not be degraded completely by trypsin or gut juice of Mythimna separata.SDS-PAGE and chromatography showed that the 130 kD protoxin was degraded to P. litura-specific toxins of 70 kD and 75 kD by P. litura gut juice, B. mori-specific toxins of 62 kD and 65 kD by B. mori gut juice and B. mori-specific toxins of 65 kD and 68 kD by trypsin. When P. lititra-specific polypeptides of 70-75 kD were subsequently treated with B. mori gut juice, the resulting polypeptides of 62-64 kD were no longer toxic to P. litura but acquired B. mori toxicity. Reversely, protoxin treated first with B. mori gut juice or trypsin and then with P. litura gut juice, resulting 62-65 kD polypeptides were toxic only to B. mori. It was shown that the action of proteases of B. mori gut juice was similar to trypsin but different from proteases of P. litura gut juice.

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