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Position: Home > Articles > Screening of Fungal Elicitor for Fermentative Production of Natamycin and Optimization of Induction Conditions FOOD SCIENCE 2013,34 (7) 196-200

促进纳他霉素合成的真菌诱导子筛选及诱导条件优化

作  者:
魏宝东;潘娅慧;王亚男;王利艳
单  位:
沈阳农业大学食品学院
关键词:
真菌诱导子;发酵;纳他霉素;响应面
摘  要:
采用真菌诱导子提高纳他霉素的产量,通过初筛,获得具有正向调节作用的真菌诱导子;采用Box-Behnken试验设计建立数学模型,进行响应面分析优化,确定诱导条件.结果表明:黑曲霉代谢产物诱导子能够有效提高纳他霉素的产量;质量浓度为122μg/mL的黑曲霉代谢产物诱导子在纳他霉素发酵18.3h后加入,诱导78.5h时,发酵液中纳他霉素的发酵产量可达到2.89g/L,为空白对照组的1.68倍.
译  名:
Screening of Fungal Elicitor for Fermentative Production of Natamycin and Optimization of Induction Conditions
单  位:
College of Food Science,Shenyang Agricultural University,Shenyang 110866,China
关键词:
fungal elicitor%fermentation%natamycin%response surface analysis
摘  要:
Preliminary screening was carried out to obtain fungal elicitor that induces natamycin production by Streptomyces chattanovgensis.The optimal induction conditions were established by mathematical modelling mathematical modelling based on Box-Behnken experimental design and response surface analysis.The potent inducing effect of the fungal elicitors on natamycin production was confirmed.When fungal elicitor from Aspergillus niger was added to a final concentration of 122 μg/mL after 18.3 h natamycin fermentation and incubated for 78.5 h,the observed average yield of natamycin in fermentation broth was 2.89 g/L,1.68 times higher than the blank control.

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