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Position: Home > Articles > Optimization of growth conditions for lactobacillus fermentum Feed Industry 2010,31 (19) 30-33

一株发酵乳杆菌培养条件的优化

作  者:
洪梅;刁其玉;闫贵龙;屠焰;张乃锋;姜成钢
单  位:
中国农业科学院饲料研究所;河北北方学院
关键词:
青贮饲料;发酵乳杆菌;培养基优化
摘  要:
为更好地提高青贮饲料的质量,研究对分离自中国青贮窖中的一株乳酸菌BLF01进行了分子学鉴定,结果显示为发酵乳杆菌。然后通过单因素试验设计研究了培养基组成(碳源、氮源)和培养条件(温度、接种量、起始pH值)对BLF01乳酸菌生长繁殖的影响。结果表明,其最适碳源是乳糖,最佳氮源是酵母粉;最佳培养条件是起始pH值6.5、培养温度35℃、接种量1%,可以考虑将BLF01作为青贮接种剂使用。
译  名:
Optimization of growth conditions for lactobacillus fermentum
作  者:
Hong Mei,Diao Qiyu,Yan Guilong,Tu Yan,Zhang Naifeng,Jiang Chenggang
关键词:
lactobacillus fermentum;culture medium;optimization
摘  要:
To improve the quality of silage and to decrease costs of microbial inoculants,culture conditions were optimized for the growth of a strain BLF01 of lactobacillus fermentum. The critical medium conditions including carbon source,nitrogen source,initial pH,temperature and inoculum size were identified by the one-factor-at-a-time approach. The optimal carbon source and nitrogen source were found to be lactose and yeast powder,respectively. The optimal pH,temperature and inoculum size for culture of BLF01 were 8.0,35 ℃ and 1%,respectively. Lactobacillus fermentum BLF01 can be used as a microbial inoculant in silage,but the effect remains to be clarified.

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