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当前位置: 首页 > 文章 > 胰淀素对小鼠脑内类固醇合成急性调节蛋白表达的影响 畜牧兽医学报 2019,50 (11) 2365-2370
Position: Home > Articles > Effect of Amylin on the Expression of Steroidogenic Acute Regulatory Protein in Mouse Brain Chinese Journal of Animal and Veterinary Sciences 2019,50 (11) 2365-2370

胰淀素对小鼠脑内类固醇合成急性调节蛋白表达的影响

作  者：
曹晓娟;李强;范奎奎;潘登;刘昊东;王昆;海日汗;杜晨光
单  位：
内蒙古农业大学职业技术学院;内蒙古农业大学兽医学院;河北省农林科学院粮油作物研究所
关键词：
脑;胰淀素;类固醇合成急性调节蛋白;蛋白表达
摘  要：
本试验旨在定位类固醇激素合成急性调节蛋白(StAR)在小鼠脑内的分布,并对胰淀素(amylin)是否影响小鼠脑内StAR的表达进行初步研究.购买C57BL/6品系小鼠,标准环境下饲养一个月后,将获得的子一代饲养7周,选取9只体重及健康状况相近的雄性小鼠为实验动物,随机分为3组:生理盐水对照组、amylin注射组和amylin+AC187(amylin特异性抑制剂)注射组,按体重(100 μg·kg-)连续注射3h后,剖取脑组织,采用免疫荧光染色技术研究StAR在脑内的分布规律,同时采用Western blot技术比较不同组中StAR的表达变化规律.试验结果表明,StAR免疫阳性信号主要表达在不定带(ZI),零星分布于蓝斑核(LC)、下丘脑腹内侧核(VMH)、中缝背核(DR).与对照组相比,腹腔注射amylin (100μg·kg-1)极显著降低ZI内StAR表达量(P<0.01);联合抑制剂处理组amylin+ AC187能极显著抑制amylin对ZI内StAR表达的影响(P<0.01).结果 表明,腹腔注射amylin可显著抑制ZI内StAR的表达,由于StAR是调节类固醇激素的限速酶,推测amylin除可调节进食及能量代谢外,还可通过调节StAR的表达影响中枢神经系统内类固醇激素的合成速率.
译  名：
Effect of Amylin on the Expression of Steroidogenic Acute Regulatory Protein in Mouse Brain
作  者：
CAO Xiaojuan;LI Qiang;FAN Kuikui;PAN Deng;LIU Haodong;WANG Kun;HAI Rihan;DU Chenguang;Vocational and Technical College, Inner Mongolia Agricultural University;College of Veterinary Medicine, Inner Mongolia Agricultural University;Institute of Grain and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences;
关键词：
brain;;amylin;;steroidogenic acute regulatory protein;;protein expression
摘  要：
This study aims to localize the distribution of steroidogenic acute regulatory(StAR) protein in the brain of small mice, and to preliminarily investigate whether amylin affects the expression of StAR in the brain of small mice. C57 BL/6 strain mice were purchased and fed under standard environment for one month. The offspring were fed for seven weeks. Nine male mice with similar weight and health status were selected as experimental animals, they were randomly divided into 3 groups: normal saline control group, amylin injection group and amylin + AC187(amylin specific inhibitor) injection group. After 3 hours' continuous injection according to body weight(100 μg·kg~(-1)), brain tissue was dissected. The distribution of StAR in brain was studied by immunofluorescence staining, and the expression of StAR in different groups was compared by Western blot. The results showed that StAR immunopositive signals were mainly expressed in zone incerta(ZI), sporadically distributed in locus coeruleus(LC), ventromedial hypothalamic nucleus(VMH) and dorsal raphe nucleus(DR). Compared with the control group, intraperitoneal injection of amylin significantly reduced the expression of StAR in ZI(P<0.01); and amylin +AC187 significantly inhibited the effect of amylin on StAR expression in ZI(P<0.01). The results showed that intraperitoneal injection of amylin could significantly inhibit the expression of StAR in ZI. StAR is a rate-limiting enzyme that regulating steroid hormones, we speculate that amylin can not only regulate food intake and energy metabolism, but also affect the synthesis rate of steroids in the central nervous system by regulating the expression of StAR.

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