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Position: Home > Articles > Tissue Culture and Plantlet Regeneration of Dendranthema indicum var. aromaticum Journal of Northeast Forestry University 2010,38 (7) 66-68

神农香菊组织培养和植株再生

作  者:
何淼;董春艳;冯博;张启翔
单  位:
北京林业大学;东北林业大学
关键词:
神农香菊;外植体;组织培养;植株再生
摘  要:
以神农香菊(Dendranthemaindicum var. aromaticum)带腋芽的茎段为外植体,研究不同种类和质量浓度生长调节物质对神农香菊茎段萌发以及再生的影响。结果表明:神农香菊茎段萌发的最佳诱导培养基为MS+6-BA0.1mg·L-1+NAA0.1mg·L-1,萌发率为90%;神农香菊茎段增殖最佳培养基为MS+6-BA0.3mg·L-1+NAA0.1mg·L-1,增殖倍数为4.1;最佳生根培养基为MS+NAA0.3mg·L-1,生根率为100%。
译  名:
Tissue Culture and Plantlet Regeneration of Dendranthema indicum var. aromaticum
作  者:
He Miao,Dong Chunyan,Feng Bo(School of Landscape Architecture,Northeast Forestry University,Harbin 150040,P. R. China); Zhang Qixiang(Beijing Forestry University)
关键词:
Dendranthema indicum var. aromaticum; Explants; Tissue culture; Plant regeneration
摘  要:
An experiment was conducted to study the effects of different concentrations and types of growth regulators on the germination and regeneration of Dendranthema indicum var. aromaticum using the stem segments with axillary buds as explants. Results show that the optimal culture medium for the stem-induced germination of D.indium var. aromaticum is MS supplemented with 0.1 mg/L 6-BA and 0.1 mg/L NAA,with a germination rate of 90%; the optimal medium for the proliferation of D.indicum var. aromaticum is the MS containing 0.3 mg/L 6-BA and 0.1 mg/L NAA,with a multiplication coefficient of 4.1; the optimal medium for rooting is MS with 0.3 mg/L NAA,with a rooting rate of 100%.

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