作 者：
魏文松;穆琳瑛;李会芹;王顺;李宏伟;侯金博;江敏;胡建东
单 位：
河南农大迅捷测试技术有限公司;河南农业大学机电工程学院;河南农业大学生命科学学院
关键词：
光学表面等离子共振;生物传感器;环境雌激素;雌二醇
摘 要：
设计了一种基于光学表面等离子共振(Surface plasmon resonance,SPR)生物传感检测系统,该生物传感检测系统由集成光学SPR生物传感器、微流池、便捷更换芯片夹具、触摸屏、信号处理电路和USB接口板组成,将该系统用于检测环境刺激中的雌二醇.先在集成光学SPR生物传感器芯片的金膜表面固定雌二醇抗原,然后分别将质量浓度为25,50,100,200 mg.L-1的雌二醇抗原添加到标准质量浓度的样品中进行预反应,将反应后的溶液流过光学SPR生物传感器芯片表面,建立了雌二醇测定标准曲线,其相关系数为0.997 8.
译 名：
Study on an optical surface plasmon resonance biosensor system for detecting estradiol in environmental estrogens
作 者：
WEI Wen-song1,MU Lin-ying1,LI Hui-qin1,WANG Shun2,LI Hong-wei2, HOU Jin-bo1,JIANG Min3,HU Jian-dong1(1.College of Mechanical and Electrical Engineering,Henan Agricultural University,Zhengzhou 450002,China;2.Henan Nongda Xunjie Measurement Technology Co.,Ltd.,Zhengzhou 450002,China; 3.College of Life Sciences,Henan Agricultural University,Zhengzhou 450002,China)
关键词：
optical surface plasmon resonance;biosensor;environmental estrogens;estradiol
摘 要：
An optical surface plasmon resonance(SPR) biosensor detection system was designed,which was used to detect estradiol in environmental estrogens and composed of an integrated optical SPR biosensor,a micro-flow cell,a clamp,a touch monitor screen and an electrical control unit(ECU) incorporated with a photoelectric conversion board and a USB interface board.In this study,the antigen-estradiol was immobilized to the surface of the biosensor chip,then the antigen-estradiol samples at 25,50,100,200 mg·L-1 were respectively added into the standard solution of antibody-estradiol for pre-reaction,and the mixture solution after pre-reaction flowed through the surface of the SPR biosensor chip,obtaining the standard curve for detecting estradiol,and coefficient of correlation is 0.997 8.
