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当前位置: 首页 > 文章 > 鱼类腐败菌腐败能力测定方法 食品科学 2010,31 (20) 355-359
Position: Home > Articles > Comparative Evaluation of Two Methods for Determining Spoilage Ability of Fish Spoilage Bacterium Shewanella putrefaciens FOOD SCIENCE 2010,31 (20) 355-359

鱼类腐败菌腐败能力测定方法

作  者：
许振伟;许钟;杨宪时;郭全友;李学英
单  位：
中国水产科学研究院东海水产研究所
关键词：
腐败菌;腐败能力;测定方法;无菌鱼块;灭菌鱼汁
摘  要：
通过分析比较接种腐败菌的大黄鱼无菌鱼块和灭菌鱼汁,在贮藏中感官、挥发性盐基氮(total volatile basenitrogen,TVBN)、三甲胺(trimethylamine,TMA)和腐败菌的变化,以及腐败菌生长动力学参数和腐败代谢产物的产量因子(YTVBN/CFU和YTMA/CFU),探讨无菌鱼块和灭菌鱼汁两种腐败能力的测定方法。结果表明:接种腐败希瓦氏菌的无菌鱼块和灭菌鱼汁的货架期分别为162h和132h,此时的TVBN含量分别为32.16mg/100g和29.64mg/100mL,TMA含量为9.31mg/100g和0.99mg/100mL,腐败希瓦氏菌菌数为8.67lg(CFU/g)和8.56lg(CFU/mL),产量因子YTVBN/CFU为2.58×10-10mg TVBN/CFU和1.98×10-10mL TVBN/CFU,产量因子YTMA/CFU为2.12×10-10mg TMA/CFU和1.27×10-11mL TMA/CFU。TMA作为接种鱼汁的理化指标是不可靠的,而TVBN可以作为接种鱼汁的理化指标。接种腐败希瓦氏菌的无菌鱼块和灭菌鱼汁产量因子YTVBN/CFU的相对误差为23.64%,因此灭菌鱼汁作为腐败菌腐败能力测定方法具有一定的可靠性。
译  名：
Comparative Evaluation of Two Methods for Determining Spoilage Ability of Fish Spoilage Bacterium Shewanella putrefaciens
作  者：
XU Zhen-wei1,2,XU Zhong1,YANG Xian-shi1,GUO Quan-you1,LI Xue-ying1 (1. East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200090,China; 2. College of Food Science,Shanghai Ocean University,Shanghai 201306,China)
关键词：
spoilage bacteria;spoilage ability;determination method;sterile fish blocks;sterilized fish juice
摘  要：
After inoculation with Shewanella putrefaciens,a common fish spoilage bacterium,the sterile block and juice of large yellow croaker were stored at (5 ± 1) ℃,and the changes in sensory quality,total volatile base nitrogen (TVBN),trimethylamine (TMA) and bacterial count,the growth kinetic parameters of this species of bacteria and the yield factors of microbial metabolites (YTVBN/CFU and YTMA/CFU) in sterile fish block and juice during the storage were comparatively measured in order to evaluate the two methods of determining the spoilage ability of Shewanella putrefaciens. The post-inoculation shelf-lives of sterile fish block and juice were 162 h and 132 h,respectively,the TVBNs 32.16 mg/100 mL and 29.64 mg/100 mL,the TMAs 9.31 mg/100 mL and 0.99 mg/100 mL,the bacterial counts 8.67 lg(CFU/mL) and 8.56 lg(CFU/mL),the YTVBN/CFU values 2.58×10-10 mg TVBN/CFU and 1.98 × 10-10 mL TVBN/CFU,and the YTMA/CFU values 2.12 × 10-10 mg TMA/CFU and 1.27 × 10-11 mL TMA/CFU at the end of shelf-life. The reliable parameter indicating the spoilage of fish juice was TVBN rather than TMA. The relative error between the YTVBN/CF values of fish block and juice was 23.64%. Thus,sterile fish juice is a promising matrix for the reliable determination of bacterial spoilage ability.

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