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当前位置: 首页 > 文章 > 八眉猪DQB基因多态性及其与仔猪腹泻的相关性研究 黑龙江畜牧兽医 2016 (15) 36-40
Position: Home > Articles > Study on the polymorphism of DQB gene in Bamei pigs and its correlation with piglet diarrhea Heilongjiang Animal Science and Veterinary Medicine 2016 (15) 36-40

八眉猪DQB基因多态性及其与仔猪腹泻的相关性研究

作  者：
王国梅;母童;金丽;张海龙;张丽;田晓静;刘丽霞
单  位：
西北民族大学生命科学与工程学院
关键词：
八眉猪;SLA-DQB;PCR-RFLP;多态性;仔猪腹泻
摘  要：
为了探讨SLA-DQB基因作为猪抗病育种辅助选择标记的可能性,试验采用PCR-RFLP方法对八眉猪SLA-DQB基因外显子2的多态性及其与仔猪腹泻的相关性进行研究。结果表明:八眉猪SLA-DQB基因外显子2多态性并不丰富,仅检测出3个等位基因和5种基因型,其多态信息含量为0.23,遗传杂合度仅为0.24;八眉猪DQB基因外显子2变异程度比较低。该群体偏离了HardyWeinberg平衡(P<0.01)。仔猪腹泻与性别不相关(P>0.05),与DQB基因外显子2的基因型之间呈现极显著相关(P<0.01),AA和AB基因型腹泻评分均极显著高于AC、BB和CC基因型(P<0.01)。不同性别DQB外显子2的基因型对仔猪腹泻的影响不同。在公猪中,AB基因型个体的腹泻评分高于AA、AC、BB、CC个体(P<0.05);而在母猪中,AA和AB腹泻评分极显著高于AC、BB和CC(P<0.01),可见性别影响了DQB外显子2基因型与腹泻之间的关系。说明八眉猪DQB基因外显子2多态性较低,其基因型对仔猪腹泻有影响,可作为猪抗病育种的选择标记。
译  名：
Study on the polymorphism of DQB gene in Bamei pigs and its correlation with piglet diarrhea
作  者：
WANG Guomei;MU Tong;JIN Li;ZHANG Hailong;ZHANG Li;TIAN Xiaojing;LIU Lixia;College of Life Science and Engineering,Northwest University for Nationalities;
关键词：
Bamei pig;;SLA-DQB;;PCR-RFLP;;polymorphism;;piglet diarrhea
摘  要：
To investigate the possibility of SLA-DQB gene as a selection-assisted marker for pig disease-resistant and breeding,the exon 2polymorphism of SLA-DQB gene and its correlation with piglet diarrhea in Bamei pigs were studied using PCR-RFLP in the test.The results showed that the exon 2 polymorphism of SLA-DQB gene in Bamei pigs was not rich,3 alleles and 5 genotypes were only detected;the polymorphic information content was 0.23,and its genetic heterozygosity was only 0.24,and the genetic variation of exon 2 of DQB gene in Bamei pigs was very low.The group deviated from the Hardy-Weinberg equilibrium(P < 0.01).The piglet diarrhea was not associated(P >0.05) with the gender of Bamei pigs,while it had highly significant correlation(P < 0.01) with the exon 2 genotype of DQB gene.The diarrhea scores of genotypes AA and AB were significantly higher(P < 0.01) than those of genotypes AC,BB and CC(P < 0.01).The exon2 genotypes of DQB gene in different genders had different effects in piglet diarrhea.in boars,The diarrhea score of individual with genotype AB was higher(P < 0.05) than that with genotypes AA,AC,BB,and CC in boars,while in the sow,the diarrhea scores of genotypes AA and AB was extremely significantly higher(P < 0.01) than those of genotypes AC,BB and CC in sows.Thus,gender influenced the relationship between the exon 2 genotype of DQB gene and diarrhea.The results indicate that the exon 2 polymorphism of DQB gene in Bamei pigs is very low,and its genotypes have an effect on piglet diarrhea,which can be used as a selectable marker of pig disease-resistant and breeding.

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