中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > IL-24对氯化钴诱导缺氧乳腺癌细胞凋亡的影响 吉林农业大学学报 2014 (3) 310-313+318
Position: Home > Articles > Effect of IL-24 on Cell Apoptosis of Hypoxia Breast Cancer Induced by Cobalt Chloride Journal of Jilin Agricultural University 2014 (3) 310-313+318

IL-24对氯化钴诱导缺氧乳腺癌细胞凋亡的影响

作  者：
王长文;马洪波;赵文秀
单  位：
吉林医药学院药学院;吉林医药学院公共卫生学院
关键词：
乳腺癌;IL-24基因;凋亡;氯化钴;缺氧
摘  要：
为研究IL-24对缺氧条件下诱导肿瘤细胞凋亡的影响。体外培养乳腺癌细胞,诱导化学缺氧模型后,转染IL-24基因,通过流式细胞术分析细胞周期和凋亡。结果表明:与对照组比较,缺氧诱导后的细胞增殖速度减慢。流式细胞术双染法分析细胞凋亡,可发现在缺氧生长条件下,IL-24基因转染可诱导乳腺癌细胞凋亡,相同时间点,细胞凋亡率无显著差异。转染IL-24基因后,缺氧和非缺氧小鼠乳腺癌细胞的红色荧光强度均随时间延长而下降。IL-24可通过内质网途径诱导缺氧以及非缺氧小鼠乳腺癌细胞发生凋亡。
译  名：
Effect of IL-24 on Cell Apoptosis of Hypoxia Breast Cancer Induced by Cobalt Chloride
作  者：
WANG Chang-wen;MA Hong-bo;ZHAO Wen-xiu;Department of Public Health,Jilin Medical College;Department of Pharmacy,Jilin Medical College;
关键词：
breast cancer;;IL-24 gene;;apoptosis;;cobalt chloride;;hypoxia
摘  要：
To analyze the effect of IL-24 on the induced tumor cell apoptosis under condition of hypoxia caused by cobalt chloride.Methods:The breast cancer cells were cultured in vitro and induced chemical anoxia models were set up.IL-24 gene transfection was carried out and cell apoptosis was analyzed by flow cytometry.The cell proliferation after hypoxia induction was slower compared with the control group.The breast cancer cell apoptosis happened after IL-24 genes transfection under condition of hypoxia by means of flow cytometry double staining.There was no significant difference of cell apoptosis rate at the same time point.The red fluorescence intensity of mouse breast cancer cells decreased with time under hypoxic and non-hypoxic conditions.IL-24 can induce the mouse breast cancer cell apoptosis under hypoxic and non-hypoxic conditions through the endoplasmic reticulum.

相似文章

导出引用
计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46