单 位：
湖南师范大学生命科学学院蛋白质化学与鱼类发育生物学教育部重点实验室
关键词：
三倍体湘云鲫;线粒体DNA;控制区;序列变异
摘 要：
对26尾三倍体湘云鲫的线粒体tRNA-Thr基因、tRNA-Pro基因和部分控制区的核苷酸序列进行了测定,获得26条长度为837—839 bp的同源基因序列,共发现65个多态性核苷酸变异位点,多态位点比例为0.077,定义了8种单元型。在湘云鲫8种单元型中确认了DNA复制终止相关的序列TAS、中央保守区序列(CSB-F、CSB-E和CSB-D)和保守序列CSB1,8种单元型含有3—5个TAS序列。在65个变异位点中,大部分序列变异为转换,8种单元型之间的序列差异在0.1%—6.3%之间。该研究为三倍体湘云鲫的繁殖和遗传改良提供了一些有价值的信息。
译 名：
VARIABILITY OF MITOCHONDRIAL DNA OF TRIPLOID HYBRIDS OF CRUCIAN CARP AND COMMON CARP
作 者：
GUO Xin-Hong,LIU Shao-Jun,Xiang Bing and LIU Yun(Key Laboratory of Protein Chemistry and Fish Developmental Biology of National Education Ministry,College of Life Sceinece,Hunan Normal University Changsha 410081)
关键词：
Triploid hybrids;Mitochondrial DNA;Control region;Sequence variation
摘 要：
The article presented the results of the first regular population study of triploid hybrids of crucian carp and common carp by sequencing the tRNA-Thr gene,tRNA-Pro gene,and partial control region of mitochondrial DNA(mtDNA).The 26 sequenced fragments ranged from 837 to 839 bp in length.The termination associated sequence(TAS) motifs,central conserved sequence blocks(CSB-F,CSB-E,CSB-D),and conserved sequence block(CSB1) were identified in the mtDNA control region.Most heteroplasmic fish had 3—5 TAS repeat units.The above sequences from 26 triploid hybrids were detected 65 polymorphic sites,yielding 8 haplotypes.Sequence divergence among different haplotypes ranged from 0.1% to 6.3%.This study provided some invaluable information for the reproducibility and genetic improvement of triploid hybrids.
