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Position: Home > Articles > Primary Study on the Composition and Structure of Polysaccharides from Bangia fusco-purpurea FOOD SCIENCE 2006,27 (11) 210-214

红毛菜多糖组成及结构的初步研究

作  者：
何碧烟;欧光南;李旭敏;陈武军;张兴如
单  位：
集美大学生物工程学院
关键词：
红毛菜;多糖;组成;结构
摘  要：
对福建产红毛菜(Bangiafusco-purpurea)的热水提取物采用Sevage法脱蛋白,乙醇沉淀得粗多糖。经DEAE-32和SephadexG-200柱层析纯化,得到3种多糖PY1、PY2和PY3,其中PY3为主要的多糖。PY3紫外光谱和理化鉴定显示它不含蛋白质和核酸;化学组成分析得出该多糖半乳糖的含量为65.9%,硫酸根含量为9.53%;元素分析表明该多糖C:N:S摩尔比约为72:2:3;该多糖的水解产物经气相色谱分析,主要由半乳糖及其衍生物组成,有少量木糖存在,半乳糖和木糖的摩尔比值为11:1;红外光谱揭示它基本不含3,6-内醚一半乳糖的特征吸收,却有较强的硫酸基吸收峰;推测该多糖可能主要由6-硫酸基-半乳糖和β-D-半乳吡喃糖为重复二糖的多糖,可能是一种原琼胶。
译  名：
Primary Study on the Composition and Structure of Polysaccharides from Bangia fusco-purpurea
作  者：
HE Bi-yan,OU Guang-nan,LI Xu-min,CHEN Wu-jun,ZHANG Xing-ru ( School of Bioengineering,Jimei University,Xiamen 361021,China)
关键词：
Bangia fusco-purpurea;polysaccharide;composition;structure
摘  要：
Polysaccharides of Bangia fusco-purpurea from Fujian province were extracted with hot water,removed proteins by method of Sevage and precipitated by ethanol. Tree purified polysaccharides were obtained by DEAE-Cellulose 32 and Sephadex G-200 column chromatography. PY3 was the main polysaccharide,which contained 65.9% galactose and 9.53% sulfate. The ultraviolet spectrum showed there were no proteins and nucleic acids in PY3. Element analysis showed the atomic ratio of C:N:S of PY3 was 72:2:3. The monomers of PY3 were galactose and xylose with a mole ratio of 11:1 by gas chromatography. And the infrared spectrum indicated that there were no specific absorption of 3,6-anhydro-galaetose but a distinct absorption of sulfate,suggested that PY3 may be a precursor agarose.

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