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Position: Home > Articles > Arctic Fox Leptin Gene Polymorphism and the Production Structure and Performance-related Analysis Chinese Agricultural Science Bulletin 2010,26 (23) 58-63

北极狐瘦素基因结构特征及其多态与生产性能相关性分析

作  者:
张敏;白秀娟
单  位:
辽宁医学院锦州畜牧兽医学院;东北农业大学动物科技学院
关键词:
北极狐;瘦素基因;多态性
摘  要:
针对实验测序得到的北极狐瘦素基因序列,利用SignalP3.0Server、ClustalX1.83、Mega4.0、Promoter predictions、TMHM2.0、ProtComp-v6.0等生物学软件和SPSS13.0软件,对其结构特征进行分析研究,并且利用PCR-SSCP的方法寻找到影响北极狐生产性能的基因位点。结果得到北极狐与狗的遗传距离最近,瘦素基因它可能是一个分泌蛋白,具有一个信号肽的序列的可能性很大,AB基因型的个体的体重、腹围、皮张长与AA、BB型个体的体重、腹围、皮张长差异显著(P<0.01),AB基因型个体的体长比AA、BB型个体的体长差异趋于显著(P<0.3),AB型基因个体的针毛长与AA、BB型个体的针毛长差异有显著的影响趋势P<0.2。表明瘦素基因突变位点导致的基因型对体重、腹围、皮张长都有极显著的影响(P<0.01)。
译  名:
Arctic Fox Leptin Gene Polymorphism and the Production Structure and Performance-related Analysis
作  者:
Zhang Min 1,Bai Xiujuan 2 (1 Jinzhou raising livestock Veterinary college Liaoning medical school,Jinzhou Liaoning 121001 ;2 College of animal science and technology,Northeast Agricultural University,Harbin 150030)
关键词:
arctic fox;leptin gene;polymorphism
摘  要:
This study on the arctic fox leptin gene structure,and through PCR-SSCP method to find the impact performance of arctic foxes loci.Data used SignalP 3.0 Server,ClustalX1.83,Mega4.0,Promoter predictions,TMHM2.0,ProtComp-v6.0 software and SPSS13.0 software such as biology to complete the analysis;arctic fox and dog genetic the distance is the latest,OB gene may be a secreted protein with a signal peptide sequence of a great possibility,AB genotype of the individual body weight,abdominal circumference,hides length and AA,BB-type individual's body weight,abdominal circumference,long hides significant difference (P<0.01),AB genotype of the body length than the AA,BB-type of individual differences in body length tended to significantly (P<0.3),AB-type genes of individual hairs length and AA,BB-type individuals The long hairs were significantly impact the trend P<0.2,showed that leptin gene mutations led to the genotype of the body weight,abdominal circumference,hides long had a significant impact on most(P<0.01).

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