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Position: Home > Articles > Single Nucleltide Polymorphism of H-FABP Gene and Correlation Analysis with Meat Quality Traits in Red Steppe Crossed Herds China Animal Husbandry & Veterinary Medicine 2008,35 (7) 67-70

草原红牛改良群体H-FABP基因SNP及其与肉质性状的相关分析

作  者:
文力正;赵玉民;姜昊;张国梁;戴立胜;赵志辉;陈承祯;张嘉保
单  位:
吉林省农业科学院;吉林大学实验动物中心;吉林大学畜牧兽医学院
关键词:
H-FABP;PCR-SSCP;SNP;肉质性状;草原红牛
摘  要:
试验采用PCR-SSCP的方法对含1/2利木赞血缘的改良草原红牛的H-FABP基因5’-调控区进行SNP分析,并分析其多态性与改良草原红牛肉质性状的相关性。结果表明:改良草原红牛H-FABP基因5’-调控区存在多态位点,在136位点发生1个T碱基的插入突变,在142位点,发生了G→A转换。相关分析结果显示剪切力在AA和AB、BB基因型间差异极显著(P<0.01),AB与BB基因型间差异不显著(P>0.05),A基因对剪切力有负效应,其余肉质性状在不同基因型间差异均不显著(P>0.05)。表明H-FABP基因对改良草原红牛肉质嫩度有显著影响。
译  名:
Single Nucleltide Polymorphism of H-FABP Gene and Correlation Analysis with Meat Quality Traits in Red Steppe Crossed Herds
作  者:
WEN Li-zheng1,ZHAO Yu-min2,JIANG Hao1,ZHANG Guo-liang2,DAI Li-sheng1,ZHAO Zhi-hui3,CHEN Cheng-zhen1,ZHANG Jia-bao1(1.Laboratory Animal Center,Jilin University,Changchun 130062,China;2.Jilin Academy of Agriculture Sciences,Changchun 130000,China;3.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China)
关键词:
H-FABP;PCR-SSCP;SNP;meat quality;Red Steppe
摘  要:
PCR-SSCP was used in this study to detect H-FABP 5'UTR polymorphisms in 16 Red Steppe hybrids crossed with Limousin.The correlationship was carried out between different genotype and meat quality.The results showed that 5'UTR of H-FABP gene exist polymorphisms in Red Steppe Crossed Herds,base T was inserted in 136 bp and the transformation of G→A in 142 bp.A genotype was negatively to the tenderness trait.The difference between AA with AB and BB was extremely significant(P<0.01) in tenderness,but AB and BB was no significant difference(P>0.05).The difference between genotype with the other meat quality was not significant(P>0.05).The effect was very significant of H-FABP gene to tenderness traits in Red Steppe Crossed Herds.

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