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Position: Home > Articles > Investigation on Polymorphism of H-FABP Gene and Relationship with Reproductive Performances in Jinhua Pig Swine Production 2005 (3) 25-27

金华猪心脏脂肪酸结合蛋白基因的多态性分析及其与繁殖性能的关系

作  者:
聂光军;彭淑红;谢爱林;林贤茂;徐宁迎
单  位:
贵州大学生物技术和环境科学学院;浙江大学动物科学学院;安徽工程科技学院生化系
关键词:
金华猪;H-FABP;繁殖性能;RFLP
摘  要:
应用PCR-RFLP方法对金华猪Ⅰ系(164头)、金华猪Ⅱ系(41头)和金华猪Ⅲ系(52头)的心脏脂肪酸结合蛋白(H-FABP)基因的1125~1817bp和1401~2216bp段进行扩增,并用HinfⅠ、HaeⅢ和MspⅠ酶分别酶切,结果表明只有HinfⅠ存在RFLP。分析不同基因型对金华猪死产数和母仔猪初生重的影响,结果表明,H-FABP基因对经产母猪的死产数影响接近极显著(P=0.015),对头胎母仔猪初生重的影响不显著(P>0.05),对经产及所有胎次的母仔猪初生重影响极显著(P<0.01)。
译  名:
Investigation on Polymorphism of H-FABP Gene and Relationship with Reproductive Performances in Jinhua Pig
作  者:
NIE Guang-jun1, PENG Shu-hong2, XIE Ai-lin3, LIN Xian-mao2, XU Ning-ying2(1.Department of Biology and Chemistry, Anhui University of Technology and Science, Wuhu 241000, China;2. College of Animal Science, Zhejiang University, Hangzhou 310029, China; 3. College of Biology Technology and Environment Science, Guizhou University, Guiyang 550025, China)
关键词:
Jinhua pig;H-FABP;reproductive performance;RFLP
摘  要:
The genotypes of 164 Jinhua pigs of line Ⅰ,41 Jinhua pigs of line Ⅱ and 52 Jinhua pigs of line Ⅲ for H-FABP locus were detected by PCR-RFLP. The PCR products were digested by enzyme HinfⅠ,Hae Ⅲand MspⅠ respectively. Only the HinfⅠcan cut the products.Effects of different genotypes of H-FABP on reproductive traits of Jinhua swine including stillbirth number and female birthweight were analyzed.The result showed that there was significant relationship between the genotype of H-FABP with reproductive traits of Jinhua pigs.

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