当前位置: 首页 > 文章 > 中华鲟和达氏鳇血清免疫球蛋白的木瓜蛋白酶水解片段分析 水产学杂志 2010,23 (3) 11-14
Position: Home > Articles > Analysis of Papain Hydrolyzed Fragments of Immunoglobulin in Serum of Chinese Sturgeon Acipenser sinesis Gray and Huso Sturgeon Georgi Chinese Journal of Fisheries 2010,23 (3) 11-14

中华鲟和达氏鳇血清免疫球蛋白的木瓜蛋白酶水解片段分析

作  者:
刘红柏;张颖;卢彤岩;王荻;赵吉伟
单  位:
中国水产科学研究院黑龙江水产研究所
关键词:
达氏鳇;中华鲟;免疫球蛋白;木瓜蛋白酶;水解片段
摘  要:
首先采用饱和硫酸氨分步沉淀和Sephadex G-200凝胶层析的方法,获得了非免疫状态下中华鲟(Acipenser sinensis Gray)和达氏鳇(Huso dauricus Georgi)的血清免疫球蛋白(Ig),在此基础上使用木瓜蛋白酶水解对所获得的免疫球蛋白片段进行了酶解,并采用SDS-PAGE和Western-blot等方法分析了所获得的水解片段。结果显示,2种鲟鱼的免疫球蛋白均可被木瓜蛋白酶水解蛋白,通过Sephadex G-100凝胶层析后均可得到两个完全分离的、均一的蛋白峰。SDS-PAGE检测两个水解片段的相对分子量分别为44KD和66KD,West-ern-blot检测结果显示,66KD的片段可以在硝酸纤维素杂交膜上被各自的兔抗鲟IgM多克隆抗体所识别,而44KD片段的检测结果为阴性。这表明,2种鲟科鱼类的木瓜蛋白酶水解特性相同,提示鲟科鱼类的免疫球蛋白在免疫学及生化方面具有较大的相似性。
译  名:
Analysis of Papain Hydrolyzed Fragments of Immunoglobulin in Serum of Chinese Sturgeon Acipenser sinesis Gray and Huso Sturgeon Georgi
作  者:
LIU Hong-bai,ZHANG Ying,LU Tong-yan,WANG Di,ZHAO Ji-wei(Heilongjiang River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Harbin 150070,China)
关键词:
Huso sturgeon;Chinese sturgeon;immunoglobulin;papain;hydrolyzed fragments
摘  要:
Saturation ammonium sulfate sedimentation and Sephadex G-200 gel chromatography were used to purify Ig from serum of Chinese sturgeon Acipenser sinesis Gray and Huso sturgeon Huso dauricus Georgi respectively,and hydrolyzed fragments by papain were analyzed.The results showed that Ig of the two fish species can be hydrolyzed into two fragments with molecule weight of 66KD and 44 KD determined by SDS-PAGE after Sephadex G-100 gel filtration.In the western blotting detection,66KD fragments can be identified by polyclonal antibody of rabbit anti sturgeon in colloxylin,while 44KD fragments cannot be deteced.A conclusion can be drawn that the hydrolyzed characters of Ig of the two fish species are same and Ig of Acipenser may have similarity in Biochemistry and Immunology.

相似文章

计量
文章访问数: 7
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊