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当前位置: 首页 > 文章 > 鹅观草属、披碱草属、猬草属和仲彬草属植物的RAPD分析及其系统学意义 四川农业大学学报 2001,19 (1) 14-20
Position: Home > Articles > Phylogenetic Relationships Among Species of Roegneria, Elymus, Hystrix and Kengyilia Based on Random Amplified Polymorphic DNA Journal of Sichuan Agricultural University 2001,19 (1) 14-20

鹅观草属、披碱草属、猬草属和仲彬草属植物的RAPD分析及其系统学意义

作  者：
周永红;郑有良;杨俊良;颜济;魏育明;杨瑞武
单  位：
四川农业大学小麦研究所
关键词：
鹅观草属;披碱草属;仲彬草属;猬草属;RAPD分析;亲缘关系;基因组
摘  要：
利用随机扩增多态性DNA(RAPD)技术分析了鹅观草属 ( 7种 )、披碱草属 ( 3种 )、猬草属 ( 2种 )和仲彬草属 ( 3种 ) 4个属的 15种植物。对 34个OPRON公司十聚体随机引物进行多态性筛选 ,2 7个 ( 79 4% )能产生多态性。 16个引物产生的 2 13个DNA片断 ,用于计算种间Nei氏遗传相似性系数分析 ,在NTSYS程序中利用UPGMA构建系统发育树状图。分析结果表明 :①这 4个属的物种各自聚类在一起 ,在相似系数 0 6水平上 ,明显分为 4组 ,分别代表了Hystrix、Elymus、Roegneria和Kengyilia 4个属 ;②Hystrix的 2个物种与Roegneria和Kengyilia物种的亲缘关系较远 ,与同具StH基因组的Elymus也有较大的RAPD变异 ;③Roegneria与Elymus亲缘关系较近 ;④Roegneria中StY和StYH基因组组合的物种亲缘关系很近 ,StY基因组的物种间存在着RAPD遗传变异 ;⑤Kengyilia与Roegneria亲缘关系较近 ;⑥RAPD结果与形态学和细胞学等分析结果一致 ,支持将它们独立作为属分类等级来处理。本文还对RAPD分析方法在小麦族系统学研究中的应用进行了讨论
译  名：
Phylogenetic Relationships Among Species of Roegneria, Elymus, Hystrix and Kengyilia Based on Random Amplified Polymorphic DNA
作  者：
ZHOU Yong hong, ZHENG You liang, YANG Jun liang, YAN Ji, WEI Yu ming, YANG Rui wu (Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan 611830, Sichuan, China)
关键词：
ROEGNERIA; ELYMUS; KENGYILIA; HYSTRIX; RAPD ANALYSIS; PHYLOGENETIC RELATIONSHIP; GENOME.
摘  要：
Fifteen species of Roegneria, Elymus, Hystrix and Kengyilia (Poaceae: Triticeae) were analyzed by using random amplified polymorphic DNA (RAPD) markers. 34 decamer oligonucleotides random primers from Opron Technologies were used for polymorphic selection. 27 (79 4%) produced polymorphic products. A total of 213 bands amplified from 16 primers were selected for RAPD analysis. The data were used to generate Nei's similarity coefficients and to construct a dendrogram by using UPGMA in NTSYS programs. It is concluded as follows: ①The species of Roegneria, Elymus, Hystrix and Kengyilia are clustered in four groups, which represent Roegneria, Elymus, Hystrix and Kengyilia respectively on 0 6 of genetic similarity coefficients; ②The relationships between the species of Hystrix and the species of Roegneria and Kengyilia are remote; ③Genetic diversity exists between Elymus and Hystrx although they possess similar StH genome; ④The species of StY and StYH genomes in Roegneria are clustered together, which suggests they have closer relationships; ⑤There is certain RAPD genetic diversity in the species of StY genomes of Roegneria; ⑥Kengyilia is related to Roegneria; ⑦RAPD results are basically comparable with those obtained from studies on morphology and cytology. The present study discusss the usefulness of RAPD markers in the systematic studies of Triticeae.

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