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Position: Home > Articles > Construction of Rare Actinomycetes Micromonospora Phage FHAU8 Genomic Library Hubei Agricultural Sciences 2007,46 (4) 16-17

稀有放线菌小单孢菌噬菌体ФHAU8基因组文库的构建

作  者:
李晓华
单  位:
中南民族大学生命科学学院生物技术国家民委重点实验室
关键词:
小单孢菌40027菌株;噬菌体;基因组文库
摘  要:
利用大肠杆菌—链霉菌穿梭质粒pHZ1358作为载体,构建了小单孢菌噬菌体ΦHAU8的基因组文库,文库质粒酶切结果表明,噬菌体ΦHAU8基因文库中的质粒DNA是由载体pHZ1358和噬菌体基因组片段组成的。
译  名:
Construction of Rare Actinomycetes Micromonospora Phage FHAU8 Genomic Library
作  者:
LI Xiao-hua(Key Laboratory of State Ethnic Affairs Commission for Biological Technology,College of Life Sciences, South-Central University for Nationalities,Wuhan 430074,China)
关键词:
Micromonospora sp.40027;phage;genomic library
摘  要:
The genomic library of ΦHAU8 was constructed with pHZ1358 used as vector.ФHAU8 genome library cosmids by digesting with BamHI and EcoRI contains fragments of pHZ1358 digested with BamHI and EcoRI,and other fragments was the same as fragments of ФHAU8 DNA digested with BamHI and EcoRI.

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