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Position: Home > Articles > Isolation and Biological Characterization of Rare Actinomycete Phage ΦSCU20 Journal of Huazhong Agricultural University 2009,28 (4) 72-75

稀有放线菌噬菌体ΦSCU20的分离及其生物学特性的研究

作  者:
艾振江;刘梅芳;何冬兰;程国军;李晓华
单  位:
中南民族大学生命科学学院/生物技术国家民委重点实验室
关键词:
噬菌体;小单孢菌40027菌株;分离;生物学特性
摘  要:
以稀有放线菌小单孢菌40027菌株为指示菌,从土壤中分离得到1株新噬菌体,命名为ΦSCU20。在测试的9株放线菌菌株中,仅能感染小单孢菌40027菌株和A-M-01菌株。电镜观察显示噬菌体ΦSCU20由多面体的头部和尾部组成。噬菌体ΦSCU20生物学特性研究表明,噬菌体ΦSCU20形成噬菌斑时培养基中适宜的Ca2+和Mg2+浓度分别为8 mmol/L和20 mmol/L;噬菌体ΦSCU20在储存液中适宜的pH值为8;噬菌体ΦSCU20不耐高温,经40℃保温30 min后,其活力降低到48.65%,经60℃保温30 min后,则完全失活;噬菌体ΦSCU20基因组特性研究表明,该噬菌体的基因组为双链DNA,基因组大小约为65 kb。
译  名:
Isolation and Biological Characterization of Rare Actinomycete Phage ΦSCU20
作  者:
AI Zhen-jiang LIU Mei-fang HE Dong-lan CHENG Guo-jun LI Xiao-hua(Key Lab for Biotechnology of the State Ethnic Affairs Commission/College of Life Science,South-Central University for Nationalities,Wuhan 430074,China)
关键词:
actinophage;Micromonospora sp.40027;isolation;biological characterization
摘  要:
Rare actinomycete phage ΦSCU20 was isolated from soil using Micromonospora sp.40027 as indicator strain.Phage ΦSCU20 could infect both Micromonospora sp.40027 and Micromonospora A-M-01.Phage ΦSCU20 has the hexagonal heads and tails;the plaque formation of phage ΦSCU20 on Micromonospora sp.40027 was the best on DNA medium with addition of 8 mmol/L Ca2+ and 20 mmol/L Mg2+;Phage ΦSCU20 was stable at pH 8;the suitable incubation temperature for the propagation of ΦSCU20 was 28 ℃;48.65% of ΦSCU20 remained viable when it was incubated at 40 ℃ for 30 min.Restriction digestion analysis of genomes of phage ΦSCU20 indicated that it was double-stranded DNA and its genome size was 65 kb.

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