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Position: Home > Articles > Diagnosis of theileriosis and 18S rDNA analysis of pathogen in cows Animal Husbandry & Veterinary Medicine 2017,49 (3) 92-95

奶牛泰勒虫病的诊断及病原体18S rDNA分析

作  者：
于伟伟;孟庆玲;乔军;卢海亭;贡莎莎;程子兵;张金生;才学鹏;陈创夫
单  位：
石河子大学动物科技学院;新疆沙湾县畜牧兽医站;中国农业科学院兰州兽医研究所;新疆塔城地区动物疾病预防控制中心
关键词：
环形泰勒虫;同源性;18S rRNA基因;系统进化树
摘  要：
采用常规血涂片镜检和PCR技术对新疆塔城地区疑似原虫感染的荷斯坦病牛进行检测。通过扩增18S rRNA目的基因并克隆测序,将测序结果与Gen Bank上的牛泰勒虫和巴贝斯虫18S r DNA参考序列对比分析同源性,并构建系统进化树,确定血液原虫的种属。血涂片鉴定结果显示,红细胞中发现圆环形或卵圆形虫体,具有牛环形泰勒虫的典型特征。同源性分析发现,塔城地区牛泰勒虫18S r DNA序列与法国牛源环形泰勒虫、欧洲南部牛源环形泰勒虫核苷酸序列同源性为99%,且遗传演化分析发现三者亲缘关系较近,证实该牛感染的虫种为环形泰勒虫。
译  名：
Diagnosis of theileriosis and 18S rDNA analysis of pathogen in cows
作  者：
YU Weiwei;MENG Qingling;QIAO Jun;LU Haiting;GONG Shasha;CHENG Zibing;ZHANG Jinsheng;CAI Xuepeng;CHEN Chuangfu;Department of Animal Science And Technology,Shihezi University;Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences;Prevention and control center of Animal disease of Tacheng District;Institute of Veterinary of Shawan County;
关键词：
Theileria annulata;;identities;;18S rRNA gene;;phylogenetic tree
摘  要：
A cow suffering a suspected blood protozoosis was diagnosed by conventional blood smear microscopy and PCR techniques in Tacheng,Xinjiang.The 18 S rRNA gene was amplified using universal primers and cloned.The sequence identities were analyzed with among the 18 S rRNA gene sequences of Theileria and Babesia reference strains from Gen Bank,and phylogenetic tree was constructed to determine the parasite species.The results of blood smear test showed that circular or oval parasites with the typical characteristics of bovine Theileria annulata were found in red blood cells.Identification of molecular biology showed that 18 S rRNA gene of Tacheng strain shared 99% identities with the nucleotide sequences of T.Annulata from French and southern Europe.Phylogenetic analysis based on 18 S rRNA genes confirmed that the Tacheng strain has close relationship with the French strain and southern Europe strain of T.annulata.The result indicated that the infected species in the cow was confirmed to be T.annulata.

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